Overwhelming proof means that in addition to the genetic adjustments of DNA mutations, epigenetic adjustments of DNA methylation and histone modifications play vital position in regulation of gene expression. DNA methylation is essentially the most frequent epigenetic alteration noticed in mammalian genomes, and usually it’s negatively correlated with gene expression. Various strategies can be found for the detection of DNA methylation adjustments. Although the current high-throughput strategies for DNA methylation evaluation have varied benefits, they require excessive ranges of technical experience, pricey gear, and reagents.
Because of these causes, many of the worldwide DNA methylation evaluation strategies are primarily carried out at core facility, and laboratories with restricted assets and experience are usually not ready to make use of these strategies. Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) is a restriction enzyme digestion and PCR-based technique for the evaluation of DNA methylation adjustments.
This technique is cost-effective, requires easy and fundamental instrumentation, and due to this fact can simply be carried out in any laboratory with fundamental setup having an everyday DNA thermal cycler and DNA gel electrophoresis system. Additional benefits of this technique over different strategies for DNA methylation evaluation are that it requires very much less quantity of DNA and might display DNA methylation adjustments globally at genome-wide stage with excessive sensitivity. This technique has been efficiently used to detect adjustments in DNA methylation both occurring naturally or induced by varied toxicants and environmental components. A element experimental protocol for MS-RAPD-PCR is described in this chapter.
Laphet is a standard fermented meals in Myanmar, constructed from tea leaves (Camellia sinensis) by fermentation with restricted air passage. We carried out microbial range analyses on 14 Laphet merchandise collected from totally different places in Myanmar. Amplicon-based sequencing outcomes revealed Lactobacillus and Acetobacter had been plentiful micro organism and Candida, Pichia, Cyberlindnera, and Debaryomyces had been plentiful yeast. Using selective media, eight species of lactic acid micro organism and 9 species of yeast had been remoted; Lactobacillus plantarum and L. collinoides had been dominant micro organism and Pichia manshurica, Candida boidinii, and Cyberlindnera jadinii had been main yeasts. PCR-DGGE evaluation confirmed that almost all of the dominant bacterial and yeast species discovered in tradition dependent evaluation had been current in Laphet samples.
High density DNA methylation array is a dependable various for PCR-based evaluation of the MGMT promoter methylation standing in glioblastoma.
MGMT promoter methylation standing is a crucial biomarker predicting survival and response to chemotherapy in sufferers affected by glioblastoma. Since new diagnostic strategies akin to methylome-based classification of mind tumors are increasingly continuously carried out, we geared toward evaluating the suitability of calculating the MGMT promoter methylation standing in a quantitative method from the methylome profiling as in comparison with the traditional gold normal evaluation by PCR.Our cohort consisted of 39 instances identified as “glioblastoma, IDH-wildtype” of which the MGMT promoter methylation standing was analyzed with each methylation-specific PCR and excessive density DNA methylation array utilizing the STP-27 algorithm.
Contradictory outcomes had been validated by pyrosequencing.The inter-method reliability reached 77% (kappa-coefficient: 0.58) when additionally instances with an inconclusive end result in one or the opposite technique had been taken into consideration. When solely instances with conclusive outcomes in each strategies had been thought of, a really excessive inter-method reliability of 91% (kappa-coefficient: 0.86) could possibly be achieved. For “methylated” instances, no contradictory outcomes had been obtained.
For the remaining two instances with discrepant outcomes subsequent pyrosequencing analyses spoke in favor of every beforehand utilized technique as soon as.In addition to its advantages for molecular subgrouping and duplicate quantity evaluation of mind tumors, DNA-methylation based mostly classification is a extremely dependable instrument for the evaluation of MGMT promoter methylation standing in glioblastoma sufferers.
PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis.
Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been a staple of analysis, offering valuable assets for molecular and genomic research. However, the most important problem is the extraction of high-quality DNA from FFPE tissues, on condition that the integrity of DNA is critically affected by formalin fixation. Formaldehyde induces crosslinks in DNA that renders single or double-stranded DNA breaks. Such breaks trigger in depth fragmentation that instantly influences the standard of DNA purified and the quantity of templates out there for PCR amplification. Thus, protocol for DNA purification from FFPE tissues should successfully extract extremely fragmented DNA and reverse cross-linking brought about by formalin fixation.
DNA extraction strategies out there in the literature had been chosen and modified at totally different phases to optimize a protocol that extracts DNA of adequate high quality and fragment dimension to be detectable by PCR. Archived FFPE tissues belonged to sufferers with triple damaging breast most cancers (TNBC) and benign breast illness had been used for the protocol optimization. The finest optimized protocol was then used to amplify Exon four area of Proviral integration web site for Moloney murine leukemia virus1 (Pim1) kinase gene to research any possible somatic mutations each in TNBCs and benign breast illnesses.
cDNA - Monkey (Rhesus) Normal Tissue: Kidney |
C1534142 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney |
C1534142-Cy |
Biochain |
40 reactions |
EUR 385 |
Total RNA - Rat Normal Tissue: Kidney |
R1434142-50 |
Biochain |
50 ug |
EUR 130 |
Genomic DNA - Rat Normal Tissue: Kidney |
D1434142 |
Biochain |
100 ug |
EUR 286 |
Rat normal kidney tissue array |
TR071a |
TissueArray |
each |
EUR 48 |
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071 |
Normal kidney and normal adjacent kidney tissue array |
KDN241a |
TissueArray |
each |
EUR 138 |
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores |
Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney |
C8235142-PP |
Biochain |
10 reactions x2 |
EUR 530 |
Kidney cancer tissue array with normal Kidney tissue |
KD2082a |
TissueArray |
each |
EUR 474 |
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082 |
Kidney Tissue Slide (Normal) |
10-401-10um |
ProSci |
10 um |
EUR 241.8 |
Kidney Tissue Slide (Normal) |
10-401-4um |
ProSci |
4 um |
EUR 216.6 |
Normal kidney tissue array |
KD803 |
TissueArray |
each |
EUR 354 |
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores |
Normal kidney tissue array |
KDN242 |
TissueArray |
each |
EUR 72 |
Description: Normal kidney tissue array, 24 cases/24 cores |
Kidney Tissue Lysate (Normal) |
1706-02 |
ProSci |
0.1 mg |
EUR 260.7 |
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
1706-03 |
ProSci |
0.1 mg |
EUR 260.7 |
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
1706-04 |
ProSci |
0.1 mg |
EUR 260.7 |
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
1706-05 |
ProSci |
0.1 mg |
EUR 260.7 |
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney cancer tissue array with adjacent normal kidney tissue |
BC07015b |
TissueArray |
each |
EUR 168 |
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a |
Total RNA - Mouse Normal Tissue: Kidney |
R1334142-50 |
Biochain |
50 ug |
EUR 130 |
Genomic DNA - Mouse Normal Tissue: Kidney |
D1334142 |
Biochain |
100 ug |
EUR 286 |
Total Protein - Mouse Normal Tissue: Kidney |
P1334142 |
Biochain |
1 mg |
EUR 188 |
Kidney cancer tissue array with matched adjacent normal kidney tissue |
KD901a |
TissueArray |
each |
EUR 258 |
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901 |
Kidney tumour with normal tissue array |
KD2001 |
TissueArray |
each |
EUR 474 |
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114 |
Total RNA - Human Adult Normal Tissue: Kidney |
R1234142-50 |
Biochain |
50 ug |
EUR 170 |
Kidney cancer tissue array with normal tissues |
KD2083 |
TissueArray |
each |
EUR 474 |
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores |
Kidney cancer tissue array with normal tissues |
KD721 |
TissueArray |
each |
EUR 168 |
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores |
Mouse Normal Tissue: Kidney Whole Cell Lysate |
MNK-100 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
Mouse Normal Tissue: Kidney Whole Cell Lysate |
MNK-50 |
Alpha Diagnostics |
50 ug |
EUR 196.8 |
Total RNA - Monkey (Rhesus) Normal Tissue: Kidney |
R1534142-50 |
Biochain |
50 ug |
EUR 191 |
Kidney tumor tissue array with normal tissue as control |
KD961 |
TissueArray |
each |
EUR 258 |
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
Total Protein - Human Adult Normal Tissue: Kidney |
P1234142 |
Biochain |
1 mg |
EUR 188 |
Kidney cancer tissue array with normal tissue as control |
KD962 |
TissueArray |
each |
EUR 258 |
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
9-80263 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney |
R1534142-Cy |
Biochain |
50 ug |
EUR 191 |
Adjacent normal kidney tissue and cancer tissue array |
BN07011b |
TissueArray |
each |
EUR 203 |
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011 |
Membrane Protein - Human Adult Normal Tissue: Kidney |
P3234142 |
Biochain |
0.1 mg |
EUR 270 |
Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue |
KD701a |
TissueArray |
each |
EUR 234 |
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701 |
FFPE Total RNA - Human Adult Normal Tissue: Kidney |
R2234142 |
Biochain |
1 ug |
EUR 744 |
Kidney cancer tissue array with matched adjacent normal tissue |
KD321a |
TissueArray |
each |
EUR 198 |
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321 |
Kidney cancer with matched adjacent normal kidney tissue array |
KD603 |
TissueArray |
each |
EUR 204 |
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores |
Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue |
Kid-060Sur01 |
TissueArray |
each |
EUR 954 |
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores |
Monkey (Cyno.) Normal Kidney Whole tissue lysate |
MCL-1190 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
Human Adult Kidney (Normal) Whole tissue lysate |
HAL-1305 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
Monkey (Rhesus) Normal Kidney Whole tissue lysate |
MRL-1301 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
Frozen Tissue Section - Human Adult Normal: Kidney |
T1234142 |
Biochain |
5 slides |
EUR 343 |
Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues |
Z7020053 |
Biochain |
5 slides |
EUR 1125.6 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
9-80075 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
9-80171 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Paraffin Tissue Section - Human Adult Normal: Kidney |
T2234142 |
Biochain |
5 slides |
EUR 185 |
Multiple Kidney cancer tissue array with normal tissue control from autopsy |
KD483 |
TissueArray |
each |
EUR 168 |
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney |
D8235142-PP-10 |
Biochain |
2x10 ug |
EUR 416 |
Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts |
9-80267 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney |
R1234142-P |
Biochain |
50 ug |
EUR 370 |
Rat Normal Kidney tissue (single section per slide) (5 slides/pack) |
RaFPT007 |
TissueArray |
5 slides |
EUR 150 |
Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack |
TS-R5405 |
IHC World |
- |
EUR 145 |
High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
KD208 |
TissueArray |
each |
EUR 546 |
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor |
D1234142 |
Biochain |
100 ug |
EUR 286 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney |
R8235142-PP-10 |
Biochain |
2x10 ug |
EUR 463 |
Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes |
9-80172 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue |
KD242 |
TissueArray |
each |
EUR 48 |
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores |
cDNA - Rat Normal Tissue: Lung |
C1434152 |
Biochain |
40 reactions |
EUR 385 |
Kidney cancer with matched cancer adjacent normal tissue test array |
KD241 |
TissueArray |
each |
EUR 48 |
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores |
Kidney cancer with matched cancer adjacent normal tissue test array |
KD241a |
TissueArray |
each |
EUR 66 |
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241 |
cDNA - Rat Normal Tissue: Brain |
C1434035 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Colon |
C1434090 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Rat Normal Tissue: Heart |
C1434122 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Liver |
C1434149 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Rectum |
C1434206 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Rat Normal Tissue: Spleen |
C1434246 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Testis |
C1434260 |
Biochain |
40 reactions |
EUR 385 |
Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells |
9-80262 |
CHI Scientific |
1 x 100 ml |
Ask for price |
cDNA - Rat Normal Tissue: Adipose |
C1434003 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Rat Normal Tissue: Bladder |
C1434010 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Stomach |
C1434248 |
Biochain |
40 reactions |
EUR 385 |
Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney |
P8235142-PP |
Biochain |
0.2 mg x2 |
EUR 663 |
cDNA - Rat Normal Tissue: Placenta |
C1434200 |
Biochain |
40 reactions |
EUR 578 |
Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes |
9-80079 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells |
9-80266 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells |
9-80269 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts |
9-80077 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts |
9-80174 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma |
KD951a |
TissueArray |
each |
EUR 1434 |
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951 |
Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack |
TS-H5013 |
IHC World |
- |
EUR 145 |
Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack |
TS-M5203 |
IHC World |
- |
EUR 145 |
Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells |
9-80078 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells |
9-80175 |
CHI Scientific |
1 x 100 ml |
Ask for price |
cDNA - Rat Normal Tissue: Skeletal Muscle |
C1434171 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Rat Normal Tissue: Small Intestine |
C1434226 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Human Tumor Tissue: Kidney |
C1235142 |
Biochain |
40 reactions |
EUR 578 |
Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk) |
TAS-1007 |
Alpha Diagnostics |
1 pk |
EUR 343.2 |
30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array |
KD805 |
TissueArray |
each |
EUR 258 |
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores |
Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells |
9-80074 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells |
9-80170 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 SLIDES/PACK) |
NCT176 |
TissueArray |
5 slides |
EUR 300 |
Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney |
T6235142-PP |
Biochain |
5 slides x2 |
EUR 583 |
Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells |
9-80076 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells |
9-80080 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells |
9-80173 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells |
9-80177 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney |
T8235142-PP |
Biochain |
5 slides x2 |
EUR 375 |
cDNA - Dog Normal Tissue: Lung |
C1734152 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Brain |
C1734035 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Cecum |
C1734089 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Heart |
C1734122 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Liver |
C1734149 |
Biochain |
40 reactions |
EUR 385 |
Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls |
T6235142-1 |
Biochain |
1 slide |
EUR 692 |
Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls |
T6235142-5 |
Biochain |
5 slides |
EUR 2273 |
cDNA - Dog Normal Tissue: Rectum |
C1734206 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Testis |
C1734260 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Lung |
C1334152 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Bladder |
C1734010 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Trachea |
C1734160 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Stomach |
C1734248 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Brain |
C1334035 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Colon |
C1334090 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Mouse Normal Tissue: Heart |
C1334122 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Liver |
C1334149 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Dog Normal Tissue: Pancreas |
C1734188 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Rectum |
C1334206 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Mouse Normal Tissue: Spleen |
C1334246 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Testis |
C1334260 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
C1634330 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
C1634360 |
Biochain |
40 reactions |
EUR 674 |
Kidney Tumor Tissue Array - Duplicated 70 cases covering all the common types of kidney cancer and 5 cases of normal and |
Z7020052 |
Biochain |
5 slides |
EUR 1657.2 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
Kidney Tumor Tissue Array - Duplicated 36 cases covering all the common types of kidney cancer and 12 cases of normal an |
Z7020054 |
Biochain |
5 slides |
EUR 1657.2 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
cDNA - Dog Normal Tissue: Esophagus |
C1734106 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Adipose |
C1334003 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Mouse Normal Tissue: Bladder |
C1334010 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Mouse Normal Tissue: Stomach |
C1334248 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
C1634390 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Human Diabetic Diseased Tissue: Kidney |
C1236142Dia |
Biochain |
40 reactions |
EUR 729 |
cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
C1634340 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
C1634350 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Mouse Normal Tissue: Placenta |
C1334200 |
Biochain |
40 reactions |
EUR 578 |
cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
C1634310 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
C1634370 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Human Adult Normal Tissue: Lung |
C1234152 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Skin |
C1234218 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Brain |
C1234035 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Colon |
C1234090 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Heart |
C1234122 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Liver |
C1234149 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Ovary |
C1234183 |
Biochain |
40 reactions |
EUR 1049 |
cDNA - Human Adult Normal Tissue: Theca |
C1234261-10 |
Biochain |
10 reactions |
EUR 215 |
cDNA - Monkey (Rhesus) Normal Tissue: Lung |
C1534152 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Monkey (Rhesus) Normal Tissue: Skin |
C1534218 |
Biochain |
40 reactions |
EUR 385 |
cDNA - Human Adult Normal Tissue: Breast |
C1234086 |
Biochain |
40 reactions |
EUR 385 |
Of the 12 totally different protocols developed, highest quality DNA in phrases of fragment dimension and purity was obtained when Tween20 lysis buffer was used for each deparaffinization and in a single day digestion together with excessive salt precipitation. Optimized protocol was then validated by extracting DNAs from 10 TNBCs and 5 benign breast illness specimens with constant purity and fragment dimension. PCR amplification and subsequent Sanger’s sequencing revealed the presence of mutations in the Exon four area of Pim1 kinase. Deparaffinization and in a single day digestion in Tween20 lysis buffer together with excessive salt precipitation yielded the highest quality PCR amplifiable DNA for mutational evaluation.