Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

Overwhelming proof means that in addition to the genetic adjustments of DNA mutations, epigenetic adjustments of DNA methylation and histone modifications play vital position in regulation of gene expression. DNA methylation is essentially the most frequent epigenetic alteration noticed in mammalian genomes, and usually it’s negatively correlated with gene expression. Various strategies can be found for the detection of DNA methylation adjustments. Although the current high-throughput strategies for DNA methylation evaluation have varied benefits, they require excessive ranges of technical experience, pricey gear, and reagents.

Because of these causes, many of the worldwide DNA methylation evaluation strategies are primarily carried out at core facility, and laboratories with restricted assets and experience are usually not ready to make use of these strategies. Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) is a restriction enzyme digestion and PCR-based technique for the evaluation of DNA methylation adjustments.

This technique is cost-effective, requires easy and fundamental instrumentation, and due to this fact can simply be carried out in any laboratory with fundamental setup having an everyday DNA thermal cycler and DNA gel electrophoresis system. Additional benefits of this technique over different strategies for DNA methylation evaluation are that it requires very much less quantity of DNA and might display DNA methylation adjustments globally at genome-wide stage with excessive sensitivity. This technique has been efficiently used to detect adjustments in DNA methylation both occurring naturally or induced by varied toxicants and environmental components. A element experimental protocol for MS-RAPD-PCR is described in this chapter.

Laphet is a standard fermented meals in Myanmar, constructed from tea leaves (Camellia sinensis) by fermentation with restricted air passage. We carried out microbial range analyses on 14 Laphet merchandise collected from totally different places in Myanmar. Amplicon-based sequencing outcomes revealed Lactobacillus and Acetobacter had been plentiful micro organism and Candida, Pichia, Cyberlindnera, and Debaryomyces had been plentiful yeast. Using selective media, eight species of lactic acid micro organism and 9 species of yeast had been remoted; Lactobacillus plantarum and L. collinoides had been dominant micro organism and Pichia manshurica, Candida boidinii, and Cyberlindnera jadinii had been main yeasts. PCR-DGGE evaluation confirmed that almost all of the dominant bacterial and yeast species discovered in tradition dependent evaluation had been current in Laphet samples.

High density DNA methylation array is a dependable various for PCR-based evaluation of the MGMT promoter methylation standing in glioblastoma.

MGMT promoter methylation standing is a crucial biomarker predicting survival and response to chemotherapy in sufferers affected by glioblastoma. Since new diagnostic strategies akin to methylome-based classification of mind tumors are increasingly continuously carried out, we geared toward evaluating the suitability of calculating the MGMT promoter methylation standing in a quantitative method from the methylome profiling as in comparison with the traditional gold normal evaluation by PCR.Our cohort consisted of 39 instances identified as “glioblastoma, IDH-wildtype” of which the MGMT promoter methylation standing was analyzed with each methylation-specific PCR and excessive density DNA methylation array utilizing the STP-27 algorithm.

Contradictory outcomes had been validated by pyrosequencing.The inter-method reliability reached 77% (kappa-coefficient: 0.58) when additionally instances with an inconclusive end result in one or the opposite technique had been taken into consideration. When solely instances with conclusive outcomes in each strategies had been thought of, a really excessive inter-method reliability of 91% (kappa-coefficient: 0.86) could possibly be achieved. For “methylated” instances, no contradictory outcomes had been obtained.

For the remaining two instances with discrepant outcomes subsequent pyrosequencing analyses spoke in favor of every beforehand utilized technique as soon as.In addition to its advantages for molecular subgrouping and duplicate quantity evaluation of mind tumors, DNA-methylation based mostly classification is a extremely dependable instrument for the evaluation of MGMT promoter methylation standing in glioblastoma sufferers.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis.

Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been a staple of analysis, offering valuable assets for molecular and genomic research. However, the most important problem is the extraction of high-quality DNA from FFPE tissues, on condition that the integrity of DNA is critically affected by formalin fixation. Formaldehyde induces crosslinks in DNA that renders single or double-stranded DNA breaks. Such breaks trigger in depth fragmentation that instantly influences the standard of DNA purified and the quantity of templates out there for PCR amplification. Thus, protocol for DNA purification from FFPE tissues should successfully extract extremely fragmented DNA and reverse cross-linking brought about by formalin fixation.

DNA extraction strategies out there in the literature had been chosen and modified at totally different phases to optimize a protocol that extracts DNA of adequate high quality and fragment dimension to be detectable by PCR. Archived FFPE tissues belonged to sufferers with triple damaging breast most cancers (TNBC) and benign breast illness had been used for the protocol optimization. The finest optimized protocol was then used to amplify Exon four area of Proviral integration web site for Moloney murine leukemia virus1 (Pim1) kinase gene to research any possible somatic mutations each in TNBCs and benign breast illnesses.

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 385

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 385

Total RNA - Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 130

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 286

Rat normal kidney tissue array

TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 530

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 130

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 286

Total Protein - Mouse Normal Tissue: Kidney

P1334142 1 mg
EUR 188

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array

KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 170

Kidney cancer tissue array with normal tissues

KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues

KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney

R1534142-50 50 ug
EUR 191

Kidney tumor tissue array with normal tissue as control

KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney

P1234142 1 mg
EUR 188

Kidney cancer tissue array with normal tissue as control

KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney

R1534142-Cy 50 ug
EUR 191

Adjacent normal kidney tissue and cancer tissue array

BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney

P3234142 0.1 mg
EUR 270

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney

R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue

KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue

Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Monkey (Cyno.) Normal Kidney Whole tissue lysate

MCL-1190 1 mg
EUR 628.8

Human Adult Kidney (Normal) Whole tissue lysate

HAL-1305 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate

MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 343

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues

Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Paraffin Tissue Section - Human Adult Normal: Kidney

T2234142 5 slides
EUR 185

Multiple Kidney cancer tissue array with normal tissue control from autopsy

KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 416

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts

9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney

R1234142-P 50 ug
EUR 370

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)

RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack

TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor

D1234142 100 ug
EUR 286

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 463

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes

9-80172 1 x 100 ml Ask for price

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue

KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 385

Kidney cancer with matched cancer adjacent normal tissue test array

KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array

KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 385

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80262 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 385

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 663

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 578

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes

9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells

9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts

9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts

9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma

KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack

TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack

TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells

9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells

9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 578

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)

TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array

KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80170 1 x 100 ml Ask for price

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 SLIDES/PACK)

NCT176 5 slides
EUR 300

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 583

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells

9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells

9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 375

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 385

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-1 1 slide
EUR 692

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-5 5 slides
EUR 2273

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 385

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

Kidney Tumor Tissue Array - Duplicated 70 cases covering all the common types of kidney cancer and 5 cases of normal and

Z7020052 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Kidney Tumor Tissue Array - Duplicated 36 cases covering all the common types of kidney cancer and 12 cases of normal an

Z7020054 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA - Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 385

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

cDNA - Human Diabetic Diseased Tissue: Kidney

C1236142Dia 40 reactions
EUR 729

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

cDNA - Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 578

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 215

cDNA - Monkey (Rhesus) Normal Tissue: Lung

C1534152 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Skin

C1534218 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 385

Of the 12 totally different protocols developed, highest quality DNA in phrases of fragment dimension and purity was obtained when Tween20 lysis buffer was used for each deparaffinization and in a single day digestion together with excessive salt precipitation. Optimized protocol was then validated by extracting DNAs from 10 TNBCs and 5 benign breast illness specimens with constant purity and fragment dimension. PCR amplification and subsequent Sanger’s sequencing revealed the presence of mutations in the Exon four area of Pim1 kinase. Deparaffinization and in a single day digestion in Tween20 lysis buffer together with excessive salt precipitation yielded the highest quality PCR amplifiable DNA for mutational evaluation.