Archives March 2021

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

Polymerase chain response (PCR) using deoxyribonucleic acid (DNA) extracted from dried blood spots (DBS) supplies a quick, cheap, and handy methodology for large-scale epidemiological research. This research in contrast the performance of PCR between DNA extracted from DBS and DNA obtained from whole blood for detecting malarial parasites. Primary research assessing the diagnostic performance of PCR using DNA extracted from DBS and whole blood for detecting malarial parasites have been obtained from the ISI Web of Science, Scopus, and PubMed databases. Odds ratios (ORs) and 95% confidence intervals (CIs) have been plotted in forest plots using Review Manager model 5.3. Statistical evaluation was carried out through random-effects meta-analysis.

Data heterogeneity was assessed using the I2 statistic. Of the 904 research retrieved from the databases, seven have been included on this research. The pooled meta-analysis demonstrated no vital distinction within the comparative performance of PCR for detecting malaria parasites between DNA extracted from DBS and that extracted from whole blood (OR 0.85; 95% CI 0.62-1.16; I2 = 78%). However, subgroup evaluation demonstrated that PCR using DNA extracted from DBS was much less correct in detecting Plasmodium vivax than that using DNA extracted from whole blood (OR = 0.85; 95% CI 0.77-0.94).

In conclusion, a vital distinction in detecting P. vivax was noticed between PCR using DNA extracted from DBS and that using DNA extracted from whole blood. Therefore, P. vivax in endemic areas ought to be recognized and detected with care with PCR using DNA obtained from DBS which doubtlessly results in a unfavourable outcome. Further research are required to research the performance of PCR using DBS for detecting P. vivax and different malarial parasites to supply knowledge in analysis and routine surveillance of malaria, particularly with renewed efforts in the direction of the eradication of the illness.

It is commonly troublesome to tell apart morphologically between intently associated species of fleas (Siphonaptera). Morphological identification of fleas usually requires microscopic examination of inner buildings in specimens cleared using caustic options. This course of degrades DNA and/or inhibits DNA extraction from specimens, which limits molecular-based research on particular person fleas and their microbiomes. Our goal was to tell apart between Oropsylla rupestris (Jordan), Oropsylla tuberculata (Baker), Oropsylla bruneri (Baker), and Oropsylla labis (Jordan & Rothschild) (Ceratophyllidae) using PCR-based single strand conformation polymorphism (SSCP) analyses and DNA sequencing. A 446 bp area of the nuclear 28S ribosomal RNA (rRNA) gene was used because the genetic marker.

Ionizing radiation, genotoxic stress, and mitochondrial DNA copy-number variation in Caenorhabditis elegans: droplet digital PCR evaluation

Mitochondria are weak to the results of ionizing radiation; harm to mitochondrial DNA (mtDNA) could also be extra intensive and persistent than harm to nuclear DNA (nDNA). Variation in mtDNA copy quantity has been proposed as a marker for mitochondrial dysfunction in response to ionizing radiation. We have developed a exact and delicate duplex droplet digital PCR (ddPCR) methodology for quantitation of the mtDNA/nDNA ratio within the mannequin organism Caenorhabditis elegans. The impact on this ratio was investigated over a big selection of doses (0.03-72 Gy) of power gamma irradiation.

Five mitochondrial targets and two nuclear reference genes have been amplified pairwise in duplex PCR format (one mitochondrial and one nuclear goal per PCR) by each ddPCR and quantitative PCR (qPCR). The outcomes confirmed that ddPCR however not qPCR enabled detection of a vital enhance in mtDNA copy quantity (1.6 ± 0.1-fold) for nematodes uncovered to excessive doses (≥24 Gy). Thus, ddPCR offered larger precision and larger sensitivity than qPCR for detection of mtDNA copy quantity variation. The variation adopted a Hill-type dose response with threshold 10.3 ± 1 Gy. This strongly means that power genotoxic stress impacts mtDNA replication. The duplex ddPCR methodology is a novel, high-precision, delicate software for willpower of mitochondrial DNA copy quantity variation and operate in C. elegans.

The outcomes obtained for 36 reference specimens (i.e., fleas that have been morphologically recognized to species) revealed no intraspecific variation in DNA sequence, whereas the DNA sequences of the 4 species of Oropsylla differed from each other at two to 6 nucleotide positions. Each flea species additionally had a distinctive SSCP banding sample. SSCP analyses have been then used to establish one other 84 fleas that had not been recognized morphologically. DNA sequencing knowledge confirmed the species id of fleas subjected to SSCP. This demonstrates that PCR-SSCP mixed with DNA sequencing of the 28S rRNA gene is a very efficient method for the delineation of 4 intently associated species of flea.

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

The diagnostic accuracy of digital PCR, ARMS and NGS for detecting KRAS mutation in cell-free DNA of sufferers with colorectal most cancers: A protocol for systematic assessment and meta-evaluation

Cetuximab and panitumumab have been used clinically to deal with metastatic colorectal most cancers for greater than 15 years. Before the remedy is given, it’s required to find out the KRAS mutation standing since it might result in drug resistance. Tumor tissue pattern is historically used for most cancers genotyping. In current years, liquid biopsy pattern has been intensively investigated as a surrogate for tumor tissue pattern resulting from its non-invasiveness and higher presentation of tumor heterogeneity.

Anti-Phospho-DNAM-1 (S329) antibody

STJ91074 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-DNAM-1 (S329).

DNAM-1 Polyclonal Antibody

ES4132-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4132-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

CD226 / DNAM-1 Antibody

20-abx119383
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNAM-1 Polyclonal Antibody

ABP53133-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53533-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

40846-100ul 100ul
EUR 252

DNAM-1 Polyclonal Antibody

40846-50ul 50ul
EUR 187

DNAM-1 Polyclonal Antibody

41787-100ul 100ul
EUR 252

DNAM-1 Polyclonal Antibody

41787-50ul 50ul
EUR 187

CD226/DNAM-1 Antibody

AF0087 200ul
EUR 304
Description: CD226/DNAM-1 antibody detects endogenous levels of total CD226/DNAM-1.

CD226/DNAM-1 Antibody

AF4778 200ul
EUR 376
Description: CD226/DNAM-1 Antibody detects endogenous levels of CD226/DNAM-1.

CD226/DNAM- 1 Antibody

ABF0087 100 ug
EUR 438

DNAM-1 Recombinant Protein

96-863 0.1 mg
EUR 490.25
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 Recombinant Protein

96-864 0.1 mg
EUR 490.25
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

DNAM-1 Polyclonal Conjugated Antibody

C40846 100ul
EUR 397

CD226/DNAM-1 Blocking Peptide

AF0087-BP 1mg
EUR 195

CD226/DNAM-1 Blocking Peptide

AF4778-BP 1mg
EUR 195

DNAM-1 / CD226 Recombinant Protein

11-431 0.1 mg
EUR 595.25
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-100ul 100ul
EUR 252

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-50ul 50ul
EUR 187

Phospho-CD226/DNAM-1 (Ser329) Antibody

AF4478 200ul
EUR 376
Description: CD226/DNAM-1 (Phospho-Ser329) Antibody detects endogenous levels of CD226/DNAM-1 only when phosphorylated at Ser329.

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Phospho-CD226/DNAM-1 (Ser329) Blocking Peptide

AF4478-BP 1mg
EUR 195

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-48T 48T
EUR 289

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-96T 96T
EUR 466

Human DNA methyltransferase,DNAM ELISA Kit

201-12-1975 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human DNA methyltransferase(DNAM)ELISA Kit

QY-E04972 96T
EUR 361

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-1mg 1mg
EUR 1318
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-500ug 500ug
EUR 963
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-50ug 50ug
EUR 278
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-1mg 1mg
EUR 1318
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-500ug 500ug
EUR 963
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-50ug 50ug
EUR 278
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-100g 100 µg
EUR 1013
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-1mg 1 mg
EUR 6402
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

DNAM-1 (Phospho-Ser329) Polyclonal Polyclonal Conjugated Antibody

C12361 100ul
EUR 397

Anti-Apaf-1 (human) Monoclonal Antibody (2E12)

M00889-1 100ug
EUR 432
Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human.

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-100g 100 µg
EUR 738
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-1mg 1 mg
EUR 4174
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-100g 100 µg
EUR 738
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-1mg 1 mg
EUR 4174
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Anti-PARP-1 Antibody

A00122-1 100ul
EUR 397
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse.

Anti-Presenilin 1 Antibody

A00138-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-CNG-1 Antibody

A05494-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Periphilin 1 Antibody

A06996-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse.

Anti-Lyl-1 Antibody

A07491-1 100ul
EUR 397
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GLI-1 Antibody

A07972-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Cerebellin 1 Antibody

A09176-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-DOC-1 Antibody

A09467-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse.

Anti-PAI-1 Antibody

A00637-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat.

Anti-Flk-1 Antibody

A00901-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse.

Anti-EDG-1 Antibody

A01502-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat.

Anti-ROBO-1 Antibody

A01530-1 100ug
EUR 455
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-NPDC-1 Antibody

A12846-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Bag-1 Antibody

A02423-1 50 ul
EUR 397
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat.

Anti-TUB 1 Antibody

A02917-1 100ug/vial
EUR 294

Anti-Dok-1 Antibody

A03039-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat.

Anti-TFIIIB90-1 Antibody

A03761-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse.

Anti-Atrophin-1 Antibody

A03828-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat.

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-48T 48T
EUR 317

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-96T 96T
EUR 496

Rat DNA methyltransferase(DNAM)ELISA Kit

QY-E10847 96T
EUR 361

Mouse DNA methyltransferase(DNAM)ELISA Kit

QY-E20479 96T
EUR 361

Anti-P-glycoprotein (human) Monoclonal Antibody (JSB-1)

M00049-1 125ug
EUR 586
Description: Mouse Monoclonal P-glycoprotein (human) Antibody (JSB-1). Validated in IF and tested in Human.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-10ug 10ug
EUR 146
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-50ug 50ug
EUR 303
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Anti-Presenilin 1 Monoclonal Antibody

M00138-1 100ug
EUR 397
Description: Rabbit Monoclonal Presenilin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Galectin 1 Monoclonal Antibody

M00470-1 100ug
EUR 397
Description: Rabbit Monoclonal Galectin 1 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DJ-1 Monoclonal Antibody

M00757-1 100ul
EUR 397
Description: Mouse Monoclonal DJ-1 Antibody. Validated in IHC, WB and tested in Bovine, Human.

Anti-Enolase 1 Monoclonal Antibody

M01250-1 100ul
EUR 397
Description: Anti-Enolase 1 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse

Anti-Dynamin 1 Monoclonal Antibody

M02536-1 100ug
EUR 397
Description: Rabbit Monoclonal Dynamin 1 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-Esrp-1 Monoclonal Antibody

M06068-1 100uL
EUR 443
Description: Mouse Monoclonal Esrp-1 Antibody. Validated in WB and tested in Human.

Anti-Angiopoietin 1/ANGPT1 Antibody

PA1333-1 100ug/vial
EUR 334

Anti-Galectin 1/LGALS1 Antibody

PB9240-1 100ug/vial
EUR 334

Anti-TNF Receptor 1 Antibody

A00294-1 200ug
EUR 522
Description: Rabbit Polyclonal TNF Receptor 1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat.

Anti-CHST8/Galnac4St 1 Antibody

A10989-1 100ul
EUR 397
Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Pyrophosphatase 1/PPA1 Antibody

A07485-1 100ug/vial
EUR 334

Anti-TCP-1 eta Antibody

A08169-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat.
The purpose of this research is to systematically summarize the accuracy of KRAS mutation measurement in colorectal most cancers using cell-free DNA in liquid biopsy samples, with tumor tissue pattern as reference (gold customary). We will search literatures within the following databases: Pubmed, Embase, and Cochrane Library. Systemic assessment and meta-analysis can be carried out to summarize the accuracy of KRAS mutation measurement in colorectal most cancers using liquid biopsy pattern, and subgroup evaluation can be carried out on totally different testing platforms, and on metastatic and non-metastatic colorectal most cancers.
Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

Cronobacter species are opportunistic pathogens succesful of inflicting life-threatening infections in people, with critical problems arising in neonates, infants, immuno-compromised people, and aged adults. The genus is comprised of seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti. Despite a multiplicity of genomic information for the genus, little is thought about seemingly transmission vectors. Using DNA microarray evaluation, in parallel with entire genome sequencing, and focused PCR analyses, the whole gene content material of two C. malonaticus, three C. turicensis, and 14 C. sakazaki remoted from numerous filth flies was assessed.

Phylogenetic relatedness amongst these and different strains obtained throughout surveillance and outbreak investigations had been comparatively assessed. Specifically, microarray evaluation (MA) demonstrated its utility to cluster strains in line with species-specific and sequence sort (ST) phylogenetic relatedness, and that the fly strains clustered amongst strains obtained from scientific, meals and environmental sources from United States, Europe, and Southeast Asia. This combinatorial method was helpful in information mining for virulence issue genes, and phage genes and gene clusters.

In addition, outcomes of plasmidotyping had been in settlement with the species id for every pressure as decided by species-specific PCR assays, MA, and entire genome sequencing. Microarray and BLAST analyses of Cronobacter fly sequence datasets had been corroborative and confirmed that the presence and absence of virulence elements adopted species and ST evolutionary traces regardless that such genes had been orthologous. In abstract, these findings assist a hanging phylogeny amongst fly, scientific, and surveillance strains remoted throughout 2010-2015, suggesting that flies are succesful vectors for transmission of virulent Cronobacter spp.; they proceed to flow into amongst United States and European populations, environments, and that this “sample of circulation” has continued over many years.

The estimation of Plasmodium falciparum parasitaemia can range in line with the technique used. Recently, droplet digital PCR (ddPCR) has been proposed as a promising method in the molecular quantitation of Plasmodium, however its potential to foretell the precise parasitaemia on scientific samples has not been largely investigated. Moreover, the risk of making use of the ddPCR-sensitive technique to serum samples has by no means been explored. Additionally, zebrafish infectivity research confirmed that these pathotypes had been as virulent to zebrafish embryos as different scientific strains.

Digital PCR-based plasma cell-free DNA mutation evaluation for early-stage pancreatic tumor prognosis and surveillance

Cell-free DNA (cfDNA) shed from tumors into the circulation presents a device for most cancers detection. Here, we evaluated the feasibility of cfDNA measurement and utility of digital PCR (dPCR)-based assays, which scale back subsampling error, for diagnosing pancreatic ductal adenocarcinoma (PDA) and surveillance of intraductal papillary mucinous neoplasm (IPMN). Hence, LAMP is a related various DNA-based amplification platform for delicate and particular detection of pathogens. The analytical sensitivity comparability amongst the typical PCR, nPCR, qPCR and LAMP reveals that the LAMP outperformed the others in phrases of LoD and amplification time.
We collected plasma from seven establishments for cfDNA measurements. Hot-spot mutations in KRAS and GNAS in the cfDNA from sufferers with PDA (n = 96), present process surveillance for IPMN (n = 112), and regular controls (n = 76) had been evaluated utilizing pre-amplification dPCR. Upon Qubit measurement and copy quantity evaluation of hemoglobin-subunit (HBB) and mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 (MT-ND1) in plasma cfDNA, HBB provided the greatest decision between sufferers with PDA relative to wholesome topics [area under the curve (AUC) 0.862], whereas MT-ND1 revealed vital variations between IPMN and controls (AUC 0.851).
DPCR using pre-amplification cfDNA afforded correct tumor-derived mutant KRAS detection in plasma in resectable PDA (AUC 0.861-0.876) and improved post-resection recurrence prediction [hazard ratio (HR) 3.179, 95% confidence interval (CI) 1.025-9.859] over that for the marker CA19-9 (HR 1.464; 95% CI 0.674-3.181). Capturing KRAS and GNAS may additionally present genetic proof in sufferers with IPMN-associated PDA and present process pancreatic surveillance.Plasma cfDNA quantification by distinct measurements is beneficial to foretell tumor burden. Through applicable strategies, dPCR-mediated mutation detection in sufferers with localized PDA and IPMN prone to progress to invasive carcinoma is possible and enhances typical biomarkers.
Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses

Loop-mediated isothermal amplification (LAMP) response as viable PCR substitute for diagnostic purposes: a comparative evaluation research of LAMP, typical PCR, nested PCR (nPCR) and real-time PCR (qPCR) primarily based on Entamoeba histolytica DNA derived from faecal pattern

This research studies the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification efficiency with typical PCR, nested PCR (nPCR) and real-time PCR (qPCR). All the assays demonstrated on this research had been developed primarily based on Serine-rich Entamoeba histolytica protein (SREHP) gene as research mannequin. A set of SREHP gene particular LAMP primers had been designed for the particular detection of Entamoeba histolytica. This set of primers recorded 100% specificity when it was evaluated in opposition to Three medically necessary Entamoeba species and 75 different pathogenic microorganisms.

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These primers had been later modified for typical PCR, nPCR and qPCR purposes. Besides, Three totally different post-LAMP analyses together with agarose gel electrophoresis, nucleic acid lateral stream immunoassay and calcein-manganese dye methods had been used to check their restrict of detection (LoD). One E. histolytica trophozoite was recorded as the LoD for all the Three post-LAMP evaluation strategies when examined with E. histolytica DNA extracted from spiked stool samples. In distinction, none of the PCR technique outperformed LAMP as each qPCR and nPCR recorded LoD of 100 trophozoites whereas the LoD of typical PCR was 1000 trophozoites.