April 2021 – Genomics Institute of Biomonitoring

Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis.

Epidermal development issue receptor (EGFR) mutation testing in plasma cell-free DNA (cfDNA) from superior lung most cancers sufferers is an rising scientific software. This meta-analysis was designed to find out the diagnostic accuracy of two widespread PCR programs, droplet digital PCR (ddPCR) and amplification refractory mutation system PCR (ARMS-PCR), for detecting EGFR mutation in cfDNA. A scientific search was carried out primarily based on PubMed, Web of science, Embase and the Cochrane library. Data from eligible research have been extracted and pooled to calculate the sensitivity, specificity, diagnostic odds ratio (DOR), space below the abstract receiver-operating attribute curve (AUROC), utilizing tissue biopsy outcomes as the usual methodology.

Subgroup analyses have been carried out relating to EGFR mutation kind, tumor stage, and EGFR-TKI remedy. Twenty-five research involving 4,881 circumstances have been included. The plasma testing sensitivity, specificity, DOR, and AUROC, in contrast with the matched tumor tissues, have been 72.1%, 95.6%, 38.5, 0.89 for ddPCR, and 65.3%, 98.2%, 52.8, 0.71 for ARMS-PCR, respectively, by oblique comparability, vital variations have been present in sensitivity (P = 0.003) and specificity (P = 0.007).

Furthermore, vital distinction was present in sensitivity between tumor stage subgroups (IIIB-IV subgroup vs. IA-IV subgroup) in ARMS-PCR (73.7 vs. 64.2%, P = 0.008), however not in ddPCR (72.5 vs. 71.2%, P = 0.756). This research demonstrates that ddPCR and ARMS-PCR have a excessive specificity with a sensible sensitivity for detecting EGFR mutation in cfDNA, which helps their utility as a complement or a conditional-alternative to tissue biopsy in scientific follow for genotyping. It appears that ddPCR has the next sensitivity than ARMS-PCR, particularly in early phases.

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Antibodies, Antibody, apoptosis, Assay Kits, Biology Cells, Blood, Blood Cells, cDNA, Clia Kits, Comprehensive genetic testing identifies targetable genomic alterations in most patients with non-small cell lung cancer, specifically adenocarcinoma, single institute investigation., Conceptualizing a Genomics Software Institute (GSI)., Culture Cells, DNA Testing, Elisa Kits, Equipments, Gels, General, Goat, Helicobacter, IgG, Immunoglobulin, Medium - Cell, Medium & Serums, Panel, PCR, Peptides, peroxidase, Plasma, plex, Rat, Real-time, Serum, Test Kits, Tissue, Whole Blood

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

by Reginald Torres April 6, 2021

Overwhelming proof means that in addition to the genetic adjustments of DNA mutations, epigenetic adjustments of DNA methylation and histone modifications play vital position in regulation of gene expression. DNA methylation is essentially the most frequent epigenetic alteration noticed in mammalian genomes, and usually it’s negatively correlated with gene expression. Various strategies can be found for the detection of DNA methylation adjustments. Although the current high-throughput strategies for DNA methylation evaluation have varied benefits, they require excessive ranges of technical experience, pricey gear, and reagents.

Because of these causes, many of the worldwide DNA methylation evaluation strategies are primarily carried out at core facility, and laboratories with restricted assets and experience are usually not ready to make use of these strategies. Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) is a restriction enzyme digestion and PCR-based technique for the evaluation of DNA methylation adjustments.

This technique is cost-effective, requires easy and fundamental instrumentation, and due to this fact can simply be carried out in any laboratory with fundamental setup having an everyday DNA thermal cycler and DNA gel electrophoresis system. Additional benefits of this technique over different strategies for DNA methylation evaluation are that it requires very much less quantity of DNA and might display DNA methylation adjustments globally at genome-wide stage with excessive sensitivity. This technique has been efficiently used to detect adjustments in DNA methylation both occurring naturally or induced by varied toxicants and environmental components. A element experimental protocol for MS-RAPD-PCR is described in this chapter.

Laphet is a standard fermented meals in Myanmar, constructed from tea leaves (Camellia sinensis) by fermentation with restricted air passage. We carried out microbial range analyses on 14 Laphet merchandise collected from totally different places in Myanmar. Amplicon-based sequencing outcomes revealed Lactobacillus and Acetobacter had been plentiful micro organism and Candida, Pichia, Cyberlindnera, and Debaryomyces had been plentiful yeast. Using selective media, eight species of lactic acid micro organism and 9 species of yeast had been remoted; Lactobacillus plantarum and L. collinoides had been dominant micro organism and Pichia manshurica, Candida boidinii, and Cyberlindnera jadinii had been main yeasts. PCR-DGGE evaluation confirmed that almost all of the dominant bacterial and yeast species discovered in tradition dependent evaluation had been current in Laphet samples.

High density DNA methylation array is a dependable various for PCR-based evaluation of the MGMT promoter methylation standing in glioblastoma.

MGMT promoter methylation standing is a crucial biomarker predicting survival and response to chemotherapy in sufferers affected by glioblastoma. Since new diagnostic strategies akin to methylome-based classification of mind tumors are increasingly continuously carried out, we geared toward evaluating the suitability of calculating the MGMT promoter methylation standing in a quantitative method from the methylome profiling as in comparison with the traditional gold normal evaluation by PCR.Our cohort consisted of 39 instances identified as “glioblastoma, IDH-wildtype” of which the MGMT promoter methylation standing was analyzed with each methylation-specific PCR and excessive density DNA methylation array utilizing the STP-27 algorithm.

Contradictory outcomes had been validated by pyrosequencing.The inter-method reliability reached 77% (kappa-coefficient: 0.58) when additionally instances with an inconclusive end result in one or the opposite technique had been taken into consideration. When solely instances with conclusive outcomes in each strategies had been thought of, a really excessive inter-method reliability of 91% (kappa-coefficient: 0.86) could possibly be achieved. For “methylated” instances, no contradictory outcomes had been obtained.

For the remaining two instances with discrepant outcomes subsequent pyrosequencing analyses spoke in favor of every beforehand utilized technique as soon as.In addition to its advantages for molecular subgrouping and duplicate quantity evaluation of mind tumors, DNA-methylation based mostly classification is a extremely dependable instrument for the evaluation of MGMT promoter methylation standing in glioblastoma sufferers.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis.

Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been a staple of analysis, offering valuable assets for molecular and genomic research. However, the most important problem is the extraction of high-quality DNA from FFPE tissues, on condition that the integrity of DNA is critically affected by formalin fixation. Formaldehyde induces crosslinks in DNA that renders single or double-stranded DNA breaks. Such breaks trigger in depth fragmentation that instantly influences the standard of DNA purified and the quantity of templates out there for PCR amplification. Thus, protocol for DNA purification from FFPE tissues should successfully extract extremely fragmented DNA and reverse cross-linking brought about by formalin fixation.

DNA extraction strategies out there in the literature had been chosen and modified at totally different phases to optimize a protocol that extracts DNA of adequate high quality and fragment dimension to be detectable by PCR. Archived FFPE tissues belonged to sufferers with triple damaging breast most cancers (TNBC) and benign breast illness had been used for the protocol optimization. The finest optimized protocol was then used to amplify Exon four area of Proviral integration web site for Moloney murine leukemia virus1 (Pim1) kinase gene to research any possible somatic mutations each in TNBCs and benign breast illnesses.

cDNA - Monkey (Rhesus) Normal Tissue: Kidney
C1534142	Biochain	40 reactions	EUR 353.5

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney
C1534142-Cy	Biochain	40 reactions	EUR 353.5

Tissue cDNA, First Strand, Rat Adult Normal, Kidney, BioGenomics
MBS652090-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Rat Adult Normal, Kidney, BioGenomics
MBS652090-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Genomic DNA - Rat Normal Tissue: Kidney
D1434142	Biochain	100 ug	EUR 241.5

Rat normal kidney tissue array
TR071a	TissueArray	each	EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney
C8235142-PP	Biochain	10 reactions x2	EUR 408.1

Normal kidney and normal adjacent kidney tissue array
KDN241a	TissueArray	each	EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Kidney cancer tissue array with normal Kidney tissue
KD2082a	TissueArray	each	EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)
10-401-10um	ProSci	10 um	EUR 241.8

Kidney Tissue Slide (Normal)
10-401-4um	ProSci	4 um	EUR 216.6

Normal kidney tissue array
KD803	TissueArray	each	EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array
KDN242	TissueArray	each	EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Slide (Normal)
MBS154338-10um	MyBiosource	10um	EUR 210

Kidney Tissue Slide (Normal)
MBS154338-4um	MyBiosource	4um	EUR 195

Kidney Tissue Slide (Normal)
MBS154338-5x10um	MyBiosource	5x10um	EUR 920

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Kidney Tissue Lysate (Normal)
1706-04	ProSci	0.1 mg	EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-05	ProSci	0.1 mg	EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-02	ProSci	0.1 mg	EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-03	ProSci	0.1 mg	EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal
GWB-F5DEBF	GenWay Biotech	0.15 mg	Ask for price

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Kidney cancer tissue array with adjacent normal kidney tissue
BC07015b	TissueArray	each	EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Genomic DNA - Mouse Normal Tissue: Kidney
D1334142	Biochain	100 ug	EUR 241.5

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Tissue Lysate (Normal) Human Kidney
GWB-FEAC28	GenWay Biotech	0.15 mg	Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue
KD901a	TissueArray	each	EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-40Tests	MyBiosource	40Tests	EUR 680

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-5x40Tests	MyBiosource	5x40Tests	EUR 2835

Kidney tumour with normal tissue array
KD2001	TissueArray	each	EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80263	CHI Scientific	1 x 100 ml	EUR 132.3

Kidney cancer tissue array with normal tissues
KD2083	TissueArray	each	EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues
KD721	TissueArray	each	EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-2Panels	MyBiosource	2Panels	EUR 805

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-5x2Panels	MyBiosource	5x2Panels	EUR 3400

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-100	Alpha Diagnostics	1 mg	EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-50	Alpha Diagnostics	50 ug	EUR 196.8

Kidney tumor tissue array with normal tissue as control
KD961	TissueArray	each	EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Kidney cancer tissue array with normal tissue as control
KD962	TissueArray	each	EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-005mg	MyBiosource	0.05mg	EUR 435

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-5x005mg	MyBiosource	5x0.05mg	EUR 1735

Adjacent normal kidney tissue and cancer tissue array
BN07011b	TissueArray	each	EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue
KD701a	TissueArray	each	EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney
R2234142	Biochain	1 ug	EUR 744

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-01mg	MyBiosource	0.1mg	EUR 575

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-5x01mg	MyBiosource	5x0.1mg	EUR 2440

Kidney cancer tissue array with matched adjacent normal tissue
KD321a	TissueArray	each	EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array
KD603	TissueArray	each	EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue
Kid-060Sur01	TissueArray	each	EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate
HAL-1305	Alpha Diagnostics	1 mg	EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate
MCL-1190	Alpha Diagnostics	1 mg	EUR 628.8

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-1mg	MyBiosource	1mg	EUR 565

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-5x1mg	MyBiosource	5x1mg	EUR 2315

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-1mg	MyBiosource	1mg	EUR 565

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-5x1mg	MyBiosource	5x1mg	EUR 2315

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5Sections	MyBiosource	5Sections	EUR 635

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5x5Sections	MyBiosource	5x5Sections	EUR 2640

Monkey (Rhesus) Normal Kidney Whole tissue lysate
MRL-1301	Alpha Diagnostics	1 mg	EUR 628.8

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-1Set	MyBiosource	1Set	EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-5x1Set	MyBiosource	5x1Set	EUR 2970

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues
Z7020053	Biochain	5 slides	EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5Sections	MyBiosource	5Sections	EUR 460

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5x5Sections	MyBiosource	5x5Sections	EUR 1925

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-01mg	MyBiosource	0.1mg	EUR 570

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-5x01mg	MyBiosource	5x0.1mg	EUR 2345

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80075	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80171	CHI Scientific	1 x 100 ml	EUR 160.65

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts
9-80267	CHI Scientific	1 x 100 ml	EUR 208.85

Multiple Kidney cancer tissue array with normal tissue control from autopsy
KD483	TissueArray	each	EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)
RaFPT007	TissueArray	5 slides	EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack
TS-R5405	IHC World	-	EUR 145

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney
D8235142-PP-10	Biochain	2x10 ug	EUR 334.6

cDNA - Rat Normal Tissue: Lung
C1434152	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Brain
C1434035	Biochain	40 reactions	EUR 317.45

cDNA - Rat Normal Tissue: Colon
C1434090	Biochain	40 reactions	EUR 467.25

cDNA - Rat Normal Tissue: Heart
C1434122	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Liver
C1434149	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Rectum
C1434206	Biochain	40 reactions	EUR 467.25

cDNA - Rat Normal Tissue: Spleen
C1434246	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Testis
C1434260	Biochain	40 reactions	EUR 311.5

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-005mg	MyBiosource	0.05mg	EUR 435

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-5x005mg	MyBiosource	5x0.05mg	EUR 1735

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-005mg	MyBiosource	0.05mg	EUR 495

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-5x005mg	MyBiosource	5x0.05mg	EUR 1995

cDNA - Rat Normal Tissue: Adipose
C1434003	Biochain	40 reactions	EUR 467.25

cDNA - Rat Normal Tissue: Bladder
C1434010	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Stomach
C1434248	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Placenta
C1434200	Biochain	40 reactions	EUR 467.25

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue
KD208	TissueArray	each	EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor
D1234142	Biochain	100 ug	EUR 241.5

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-01mg	MyBiosource	0.1mg	EUR 575

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-5x01mg	MyBiosource	5x0.1mg	EUR 2440

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes
9-80172	CHI Scientific	1 x 100 ml	EUR 160.65

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80262	CHI Scientific	1 x 100 ml	EUR 132.3

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue
KD242	TissueArray	each	EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241	TissueArray	each	EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241a	TissueArray	each	EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells
9-80266	CHI Scientific	1 x 100 ml	EUR 132.3

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80269	CHI Scientific	1 x 100 ml	EUR 132.3

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-005mg	MyBiosource	0.05mg	EUR 485

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-5x005mg	MyBiosource	5x0.05mg	EUR 1960

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-1Kit	MyBiosource	1Kit	EUR 730

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-5x1Kit	MyBiosource	5x1Kit	EUR 3075

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-1Set	MyBiosource	1Set	EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-5x1Set	MyBiosource	5x1Set	EUR 2970

cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171	Biochain	40 reactions	EUR 311.5

cDNA - Rat Normal Tissue: Small Intestine
C1434226	Biochain	40 reactions	EUR 467.25

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes
9-80079	CHI Scientific	1 x 100 ml	EUR 160.65

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
9-80077	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts
9-80174	CHI Scientific	1 x 100 ml	EUR 208.85

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma
KD951a	TissueArray	each	EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack
TS-H5013	IHC World	-	EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack
TS-M5203	IHC World	-	EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells
9-80078	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells
9-80175	CHI Scientific	1 x 100 ml	EUR 160.65

Tissue, Section, Matched Pairs, Human Primary Tumor and Normal, Kidney, Frozen
MBS640478-2x5Sections	MyBiosource	2x5Sections	EUR 900

Tissue, Total Protein, Matched Pairs, Human Primary Tumor and Normal, Kidney
MBS656969-2x02mg	MyBiosource	2x0.2mg	EUR 935

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)
TAS-1007	Alpha Diagnostics	1 pk	EUR 343.2

Tissue, Array, Rat Adult Normal, Multi, tissue I, heart, brain, kidney, liver, lung, spleen, small intestine, skeletal muscle
MBS639715-5Arrays	MyBiosource	5Arrays	EUR 510

Tissue, Array, Rat Adult Normal, Multi, tissue I, heart, brain, kidney, liver, lung, spleen, small intestine, skeletal muscle
MBS639715-5x5Arrays	MyBiosource	5x5Arrays	EUR 2145

Tissue, Section, Matched Pairs, Human Primary Tumor and Normal, Kidney (Paraffin)
MBS640058-2x5Sections	MyBiosource	2x5Sections	EUR 660

Tissue, Array, Human Tumor, Same Types of Tumor, Kidney Tumor, kidney tumors x3, normal control x1, normal placenta (matched with mRNA blots) (Paraffin)
MBS639605-5Arrays	MyBiosource	5Arrays	EUR 660

Tissue, Array, Human Tumor, Same Types of Tumor, Kidney Tumor, kidney tumors x3, normal control x1, normal placenta (matched with mRNA blots) (Paraffin)
MBS639605-5x5Arrays	MyBiosource	5x5Arrays	EUR 2810

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80074	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80170	CHI Scientific	1 x 100 ml	EUR 160.65

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array
KD805	TissueArray	each	EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

cDNA - Human Tumor Tissue: Kidney
C1235142	Biochain	40 reactions	EUR 467.25

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 slides/pack)
NCT176	TissueArray	5 slides	EUR 300

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells
9-80076	CHI Scientific	1 x 100 ml	EUR 160.65

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells
9-80080	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells
9-80173	CHI Scientific	1 x 100 ml	EUR 160.65

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80177	CHI Scientific	1 x 100 ml	EUR 160.65

cDNA - Dog Normal Tissue: Lung
C1734152	Biochain	40 reactions	EUR 311.5

cDNA - Dog Normal Tissue: Brain
C1734035	Biochain	40 reactions	EUR 317.45

cDNA - Dog Normal Tissue: Cecum
C1734089	Biochain	40 reactions	EUR 311.5

cDNA - Dog Normal Tissue: Heart
C1734122	Biochain	40 reactions	EUR 311.5

cDNA - Dog Normal Tissue: Liver
C1734149	Biochain	40 reactions	EUR 311.5

cDNA - Dog Normal Tissue: Rectum
C1734206	Biochain	40 reactions	EUR 311.5

cDNA - Dog Normal Tissue: Testis
C1734260	Biochain	40 reactions	EUR 311.5

cDNA - Mouse Normal Tissue: Lung
C1334152	Biochain	40 reactions	EUR 311.5

Of the 12 totally different protocols developed, highest quality DNA in phrases of fragment dimension and purity was obtained when Tween20 lysis buffer was used for each deparaffinization and in a single day digestion together with excessive salt precipitation. Optimized protocol was then validated by extracting DNAs from 10 TNBCs and 5 benign breast illness specimens with constant purity and fragment dimension. PCR amplification and subsequent Sanger’s sequencing revealed the presence of mutations in the Exon four area of Pim1 kinase. Deparaffinization and in a single day digestion in Tween20 lysis buffer together with excessive salt precipitation yielded the highest quality PCR amplifiable DNA for mutational evaluation.

Universal DNA Marker (100 bp ~ 10,000 bp)
W2583-500	101Bio	500 rxn	EUR 399

Universal Genomic DNA Kit
MBS278033-200Preps	MyBiosource	200Preps	EUR 380

Universal Genomic DNA Kit
MBS278033-50Preps	MyBiosource	50Preps	EUR 215

QPCR Kit DNA Universal f - EACH
MOL6606	Scientific Laboratory Supplies	EACH	EUR 1258.76

QPCR Kit DNA Universal m - EACH
MOL6610	Scientific Laboratory Supplies	EACH	EUR 1258.76

Genorise® Universal DNA Mini Kit 50
GR101076	Genorise Scientific	50	EUR 223

Genorise® Universal DNA Mini Kit 100
GR101076-100	Genorise Scientific	100	EUR 357

Genorise® Universal DNA Mini Kit 200
GR101077	Genorise Scientific	200	EUR 567

Genorise® Universal DNA Mini Kit 500
GR101077-500	Genorise Scientific	500	EUR 1272

Universal Genomic DNA Extraction Kit
D2100	Solarbio Life Science	Ask for price Ask for price	50 Tests 100 Tests

Methylamp Universal Methylated DNA Kit
P-1011	EpiGentek	Ask for price Ask for price Ask for price	20 Modifications 10 Modifications 20 Modifications

Allprep DNA/RNA/miRNA Universal Kit
2148A	Unibiotest	50 Tests	EUR 500

Allprep DNA/RNA/miRNA Universal Kit
2148B	Unibiotest	4*50 Tests	EUR 1800

Universal Methylated Human DNA Standard
D5011	Zymo Research	1 PC	Ask for price

Universal Methylated Mouse DNA Standard
D5012	Zymo Research	1 PC	Ask for price

VAHTS Universal DNA Library Prep Kit for MGI
NDM607-01	Vazyme	24 rxn	EUR 879.6

VAHTS Universal DNA Library Prep Kit for MGI
NDM607-02	Vazyme	96 rxn	EUR 2563.2

Universal Solution for DNA isolation (GeDI)
E3760-01	EURx	25ml	EUR 30.52

Universal Solution for DNA isolation (GeDI)
E3760-02	EURx	100ml	EUR 95.92

QPCR Kit DNA Universal f Mmix - EACH
MOL6608	Scientific Laboratory Supplies	EACH	EUR 1581.9

QPCR Kit DNA Universal m Mmix - EACH
MOL6612	Scientific Laboratory Supplies	EACH	EUR 1581.9

Quick DNA Universal Microprep Kit (50 Preps)
D4074	Zymo Research	50 Preparations	Ask for price

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-01-24rxns	Vazyme	24 rxns	EUR 304.66

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-02-96rxns	Vazyme	96 rxns	EUR 1139.05

VAHTS Universal DNA library Prep Kit for MGI V4
NDM610-01	Vazyme	24 rxns	EUR 235.2

VAHTS Universal DNA library Prep Kit for MGI V4
NDM610-02	Vazyme	96 rxns	EUR 880

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit
E3597-01	EURx	25preps	EUR 81.75
Description: Kit for isolation of total DNA/RNA/Protein from the same biological sample(bacteria, tissue, plant, yeast, cell culture)

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit
E3597-02	EURx	100preps	EUR 299.75
Description: Kit for isolation of total DNA/RNA/Protein from the same biological sample(bacteria, tissue, plant, yeast, cell culture)

Archives April 2021

Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis.

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity analysis and complementing forensic surveillance.

Universal DNA Marker (100 bp ~ 10,000 bp)

Universal Genomic DNA Kit

Universal Genomic DNA Kit

QPCR Kit DNA Universal f - EACH

QPCR Kit DNA Universal m - EACH

Genorise® Universal DNA Mini Kit 50

Genorise® Universal DNA Mini Kit 100

Genorise® Universal DNA Mini Kit 200

Genorise® Universal DNA Mini Kit 500

Universal Genomic DNA Extraction Kit

Methylamp Universal Methylated DNA Kit

Allprep DNA/RNA/miRNA Universal Kit

Allprep DNA/RNA/miRNA Universal Kit

Universal Methylated Human DNA Standard

Universal Methylated Mouse DNA Standard

QPCR Kit DNA Universal f Mmix - EACH

QPCR Kit DNA Universal m Mmix - EACH

VAHTS Universal DNA Library Prep Kit for MGI

VAHTS Universal DNA Library Prep Kit for MGI

Universal Solution for DNA isolation (GeDI)

Universal Solution for DNA isolation (GeDI)

Quick DNA Universal Microprep Kit (50 Preps)

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V4

VAHTS Universal DNA library Prep Kit for MGI V4

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit

Allprep RNA/DNA/miRNA/protein Universal Kit

Allprep RNA/DNA/miRNA/protein Universal Kit

Methylamp Universal Methylated DNA Preparation Kit

VAHTS Universal DNA Library Prep Kit for Illumina V3

VAHTS Universal DNA Library Prep Kit for Illumina V3

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Ion Torrent

VAHTS Universal DNA Library Prep Kit for Ion Torrent

AFTSpin Universal Plant Fast DNA Extraction Kit

Mag Beads Universal Gel DNA Extraction Kit ⭐

Universal Genomic DNA Purification Mini Spin Kit

Universal Genomic DNA Purification kit（Spin Column)

Universal Genomic DNA Purification kit（Spin Column)

EpiMag High Throughput DNA Isolation Universal Kit

EpiMag High Throughput DNA Isolation Universal Kit

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

VAHTS Universal Pro DNA Library Prep Kit for MGI

VAHTS Universal Pro DNA Library Prep Kit for MGI

Universal Kit for DNA isolation with GeDI Solution

Universal Kit for DNA isolation with GeDI Solution

VAHTS Universal Plus DNA Library Prep Kit for MGI

VAHTS Universal Plus DNA Library Prep Kit for MGI

VAHTS Universal DNA Library Prep Kit for Illumina V4 (Plate)

VAHTS Universal Pro DNA Library Prep Kit for Illumina

VAHTS Universal Pro DNA Library Prep Kit for Illumina

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina

VAHTS Universal Plus DNA Library Prep Kit for Illumina

Bisulfite-Converted Universal Methylated Human DNA Standard

Universal Shaker

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

Universal Platform

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

Allprep RNA/DNA/miRNA/protein Universal Kit
2149A	Unibiotest	50 Tests	EUR 600

Allprep RNA/DNA/miRNA/protein Universal Kit
2149B	Unibiotest	4*50 Tests	EUR 2200

Methylamp Universal Methylated DNA Preparation Kit
P-1019	EpiGentek	40 µg	EUR 416.7

VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-01	Vazyme	24 rxn	EUR 529.2

VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-02	Vazyme	96 rxn	EUR 1686

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-01	Vazyme	24 rxns	EUR 294

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-01-24rxns	Vazyme	24 rxns	EUR 304.44

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-02	Vazyme	96 rxns	EUR 1100

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-02-96rxns	Vazyme	96 rxns	EUR 1139.05