Archives May 2021

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

The research of populations of massive dimension and excessive variety is proscribed by the aptitude of gathering knowledge. Moreover, for a pool of people, every related to a distinctive attribute function, because the pool dimension grows, the potential interactions enhance exponentially, rapidly past the restrict of computation and experimental research. Herein, we current designs of DNA libraries with numerous variety. Using a facile analytic technique primarily based on actual time PCR, we are able to consider the range of a pool of DNA permitting terribly excessive heterogenicity (e.g. > 1 trillion).

We demonstrated that these DNA libraries can be utilized to mannequin heterogeneous populations, exhibiting capabilities corresponding to self-protection, appropriate for biased enlargement, and to evolve into amorphous constructions. The technique has proven the exceptional energy of parallel computing utilizing DNA, as it may well resemble an analogue pc and be utilized in selection-based biotechnology strategies, corresponding to DNA-encoded chemical libraries. As a chemical method to unravel issues historically for genetic and statistical evaluation, the strategy offers a fast and cost-efficient analysis of library variety for the intermediate steps by a choice course of.

Consistent variations amongst melting curves of PCR-amplified DNA fragments are handled by normalizing the relative fluorescence models (RFU) and performing a clustering evaluation, however statistically important variations amongst curves usually are not often decided. In the current research, an evaluation primarily based on useful knowledge evaluation (FDA) was applied to judge the existence of statistically important variations between normalized RFU curves obtained from PCR-HRM (high-resolution melting) evaluation by utilizing ANOVA for useful knowledge.

The effectiveness of the FDA technique was analyzed with knowledge from a set of samples of eight animal species of curiosity in meals evaluation, in addition to mixtures of DNA from these species, analyzed by PCR-HRM to distinguish them. The statistical technique described on this research has been demonstrated to be a sturdy and exact device to discriminate amongst melting curves derived from HRM evaluation. This technique has benefits over the present comparability strategies. PRACTICAL APPLICATION: As lengthy as meals fraud and mislabeling exist, new methods for species identification are wanted.

Rapid and Reliable One-Step ABO Genotyping Using Direct Real-Time Allele-Specific PCR and Melting Curve Analysis Without DNA Preparation.

ABO genotyping is a molecular diagnostic method necessary for transfusion and transplantation in medication, and human identification in forensic science. Because ABO genotyping are labor intensive and time consuming, the genotyping can’t be firstly used to resolve the serological ABO discrepancy in blood financial institution. For fast one-step ABO genotyping, we developed direct, real-time, allele-specific polymerase chain response (PCR), and melting curve evaluation (DRAM assay) with out DNA preparation. In DRAM assay, we used a particular PCR buffer for direct PCR, a fast RBC lysis buffer, white blood cells as template with out DNA preparation, allele-specific primers for discriminating three ABO alleles (261G/del, 796C/A, and 803G/C), and melting curve evaluation as a detection technique.

There was 100% concordance among the many outcomes of ABO genotyping by the DRAM assay, serologic typing, PCR-RFLP and PCR-direct sequencing of 96 venous blood samples. We have been capable of cut back the quantity of handbook steps to a few and the hands-on time to 12 min, in comparison with seven steps and roughly 40 min for standard ABO genotyping utilizing allele-specific PCR with purified DNA and agarose gel electrophoresis. We have established and validated the DRAM assay for fast and dependable one-step ABO genotyping in a closed system. The DRAM assay with an acceptable quantity of allele-specific primers may assist in resolving ABO discrepancies and ought to be helpful in scientific laboratory and blood financial institution.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analyses of the genetic variety and inhabitants constructions of Histoplasma capsulatum scientific isolates from Mexico, Guatemala, Colombia and Argentina, utilizing a randomly amplified polymorphic DNAPCR assay.

We studied the genetic variety and the inhabitants construction of human isolates of Histoplasma capsulatum, the causative agent of histoplasmosis, utilizing a randomly amplified polymorphic DNA-polymerase chain response (RAPD-PCR) assay to determine associations with the geographic distribution of isolates from Mexico, Guatemala, Colombia and Argentina. The RAPD-PCR sample analyses revealed the genetic variety by estimating the proportion of polymorphic loci, efficient quantity of alleles, Shannon’s index and heterozygosity. Population construction was recognized by the index of affiliation (IA) take a look at. These knowledge contribute to the information on the molecular epidemiology of histoplasmosis in Latin America.
Thirty-seven isolates have been studied and clustered into three teams by the unweighted pair-group technique with arithmetic imply (UPGMA). Group I contained 5 subgroups primarily based on geographic origin. The consistency of the UPGMA dendrogram was estimated by the cophenetic correlation coefficient (CCCr = 0.94, P = 0.001). Isolates from Mexico and Colombia introduced larger genetic variety than isolates from Argentina. Isolates from Guatemala grouped along with the reference strains from the United States of America and Panama. The IA values recommend the presence of a clonal inhabitants construction within the Argentinian H. capsulatum isolates and additionally validate the presence of recombining populations within the Colombian and Mexican isolates.

Mouse Submandibular gland protein C, Muc19 ELISA KIT

ELI-35955m 96 Tests
EUR 865

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m 96 Tests
EUR 865

Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT

ELI-06139r 96 Tests
EUR 886

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 ea Ask for price

Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL

ELI-27901r 96 Tests
EUR 886

Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K

EF017179 96 Tests
EUR 689

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

Evo™ cDNA Kit

M1164-100 Ask for price

Evo™ cDNA Kit

M1164-25 Ask for price

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 134

amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
EUR 192

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 415

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 445

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 415

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 415

Human Adult cDNA Tissue: Testis

HA-260 10 rxn
EUR 415

Human Tumor Tissue: Breast cDNA

HT05-090 10 rxn
EUR 415

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 77.76

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Decoding DNA labels by melting curve analysis using real-time PCR.

Decoding DNA labels by melting curve analysis using real-time PCR.

Synthetic DNA has been used as an authentication code for a various variety of purposes. However, current decoding approaches are primarily based on both DNA sequencing or the willpower of DNA size variations. Here, we current a easy different protocol for labeling totally different objects using a small variety of brief DNA sequences that differ of their melting factors. Code amplification and decoding will be carried out in two steps using quantitative PCR (qPCR). To acquire a DNA barcode with excessive complexity, we outlined Eight template teams, every having four totally different DNA templates, yielding 158 (>2.5 billion) combos of various particular person melting temperature (Tm) values and corresponding ID codes.

The reproducibility and specificity of the decoding was confirmed by using probably the most advanced template combination, which had 32 totally different merchandise in Eight teams with totally different Tm values. The industrial applicability of our protocol was additionally demonstrated by labeling a drone with an oil-based paint containing a predefined DNA code, which was then efficiently decoded. The methodology introduced right here consists of a easy code system primarily based on a small variety of artificial DNA sequences and a cheap, fast decoding protocol using a couple of qPCR reactions, enabling a variety of authentication purposes.

Current research investigated the anti-mosquito potential of Achyranthes aspera towards the dengue vector, Aedes aegypti. The stems and leaves of A. aspera have been extracted in hexane and evaluated for his or her toxicity towards early fourth instars of A. aegypti. The larvicidal efficacy of the extract was validated as per WHO protocol. The mortality counts have been made after 24 h and LC values have been calculated at totally different ranges. No important variations in cfDNA concentrations have been detected between numerous time factors of as much as 24 h till centrifugation.

The antagonistic affect of extracts was additionally explored on the larval genomic DNA. The larvae have been uncovered to extracts at LC50 ranges and the alterations in g-DNA was evaluated via RAPD-PCR method using three random primers; MA-09, MA-12 and MA-26. Our investigations ascertained the larvicidal efficacy of each the leaf and stem extracts of A. aspera leading to respective LC50 values of 0.068 and 0.082 mg/mL. The extracts additionally precipitated variable genotoxic results with important adjustments within the RAPD profiles.

Digital PCR analysis of circulating tumor DNA: a biomarker for chondrosarcoma prognosis, prognostication, and residual illness detection.

Conventional chondrosarcoma is the most typical main bone tumor in adults. Prognosis corresponds with tumor grade however stays variable, particularly for people with grade (G) II illness. There are at the moment no biomarkers out there for monitoring or prognostication of chondrosarcoma. Circulating tumor DNA (ctDNA) has lately emerged as a promising biomarker for a broad vary of tumor sorts. To date, little has been carried out to review the presence of ctDNA and its potential utility within the administration of sarcomas, together with chondrosarcoma.

In this research, we’ve assessed ctDNA ranges in a cohort of 71 sufferers, 32 with sarcoma, together with 29 people with central chondrosarcoma (CS) and 39 with domestically aggressive and benign bone and gentle tissue tumors, using digital PCR. In sufferers with CS, ctDNA was detected in pretreatment samples in 14/29 sufferers, which confirmed clear correlation with tumor grade as demonstrated by the detection of ctDNA in all sufferers with GIII and dedifferentiated illness (n = 6) and in 8/17 sufferers with GII illness, however by no means related to GI CS. Notably detection of ctDNA preoperatively in GII illness was related to a poor final result.

A complete of 14 sufferers with CS had ctDNA ranges assessed at a number of time factors and in most sufferers there was a transparent discount following surgical elimination. This analysis lays the inspiration for bigger research to evaluate the utility of ctDNA for chondrosarcoma prognosis, prognostication, early detection of residual illness and monitoring illness development. DdPCR outcomes on cfDNA are extremely depending on a number of elements throughout preanalytical pattern workup, which have to be addressed through the growth of this diagnostic software for most cancers diagnostics sooner or later.

Decoding DNA labels by melting curve analysis using real-time PCR.

Preanalytical blood pattern workup for cell-free DNA analysis using Droplet Digital PCR for future molecular most cancers diagnostics.

In present molecular most cancers diagnostics, using blood samples of most cancers sufferers for the detection of genetic alterations in plasma (cell-free) circulating tumor DNA (ctDNA) is an rising observe. Since ctDNA ranges in blood are low, extremely delicate Droplet Digital PCR (ddPCR) can be utilized for detecting uncommon mutational targets. In order to carry out ddPCR on blood samples, a standardized process for processing and analyzing blood samples is critical to facilitate implementation into scientific observe. Therefore, we assessed the technical pattern workup process for ddPCR on blood plasma samples.

Genomic DNA from Human Tumor Tissue: Uterus Tumor, from a single donor

D1235274 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Adult Normal Tissue: Uterus: Cervix, from a single donor

D1234275 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA Kit

20-abx098077
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242
  • EUR 105.00
  • EUR 328.00
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 272

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 398

Animal Genomic DNA Kit

abx294005-100preps 100 preps
EUR 523

Animal Genomic DNA Kit

abx294005-50preps 50 preps
EUR 398

Human Brain Genomic DNA

X11001 10 µg Ask for price
Description: kits suitable for this type of research

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 321

Soil Genomic DNA Kit

K1411-250
EUR 561

Soil Genomic DNA Kit

K1411-50
EUR 316

Insect Genomic DNA Kit

K1412-250
EUR 561

Insect Genomic DNA Kit

K1412-50
EUR 316

Mollusc Genomic DNA Kit

K1413-250
EUR 561

Mollusc Genomic DNA Kit

K1413-50
EUR 316

Yeast Genomic DNA Kit

K1414-250
EUR 561

Yeast Genomic DNA Kit

K1414-50
EUR 316

Fungal Genomic DNA Kit

K1415-250
EUR 561

Fungal Genomic DNA Kit

K1415-50
EUR 316

Genomic DNA Isolation Kit

K281-50
EUR 272

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA - Human Male

D1234999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male

D1434999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female

D1434999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male

D1734999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male

D1834999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male

D1934999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male

D1B34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male

D1C34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male

D1H34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male

D1O34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Extraction Kit

K5016005 1 kit
EUR 329

Plant Genomic DNA extraction buffer

20-abx098075
  • EUR 286.00
  • EUR 105.00
  • 100 ml
  • 1.2 ml

Marine Animal Genomic DNA Kit

20-abx098079
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns

FFPE Tissue Genomic DNA Kit

abx098866-50rxns 50 rxns
EUR 272

Genomic DNA Extraction and Purification

EP10012 100 Tests
EUR 505

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P 50 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P 25 Preps Ask for price

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 550

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

AccuRT Genomic DNA Removal Kit

G488 200 Reactions
EUR 82

Bacterial Genomic DNA Isolation Kit

K309-100
EUR 376

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male

D1534999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male

D1G34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 250 prep
EUR 536

Genomic DNA from Mouse Normal Tissue: Brain

D1334035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Heart

D1334122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Liver

D1334149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Lung

D1334152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Spleen

D1334246 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Stomach

D1334248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 76.64

Rapid Animal Genomic DNA Extraction Kit

AT4780 10Preps
EUR 69.03

Rapid Animal Genomic DNA Extraction Kit

AT4781 50Preps
EUR 126.13

Rapid Animal Genomic DNA Extraction Kit

AT4782 250preps
EUR 354.5

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 76.64

Rapid Yeast Genomic DNA Extraction Kit

BS8227 50Preps
EUR 76.64

Rapid Blood Genomic DNA Extraction Kit

BT4782 50Preps
EUR 76.64

Genomic DNA Kit (No RNase A)

20-abx098862
  • EUR 398.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price
Description: The best epigenetics products

Plant Tissue Genomic DNA Isolation Kit

K316-100
EUR 376

Mammalian Cell Genomic DNA Isolation Kit

K967-100
EUR 381

Genomic DNA from Mouse Normal Tissue: Skeletal Muscle

D1334171 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Lysate

1317 0.1 mg
EUR 191
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 100 preps
EUR 165

Blood samples from wholesome people, in addition to lung most cancers sufferers have been analyzed. We in contrast totally different strategies and protocols for pattern assortment, storage, centrifugation, isolation, and quantification. Cell-free DNA (cfDNA) concentrations of a number of wild-type targets and BRAF and EGFR-mutant ctDNA concentrations quantified by ddPCR have been main final result measurements. Highest cfDNA concentrations have been measured in blood collected in serum tubes.  Highest cfDNA concentrations have been detected after DNA isolation with the Quick cfDNA Serum & Plasma Kit, whereas plasma isolation using the QIAamp Circulating Nucleic Acid Kit yielded probably the most constant outcomes.