Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

The research of populations of massive dimension and excessive variety is proscribed by the aptitude of gathering knowledge. Moreover, for a pool of people, every related to a distinctive attribute function, because the pool dimension grows, the potential interactions enhance exponentially, rapidly past the restrict of computation and experimental research. Herein, we current designs of DNA libraries with numerous variety. Using a facile analytic technique primarily based on actual time PCR, we are able to consider the range of a pool of DNA permitting terribly excessive heterogenicity (e.g. > 1 trillion).

We demonstrated that these DNA libraries can be utilized to mannequin heterogeneous populations, exhibiting capabilities corresponding to self-protection, appropriate for biased enlargement, and to evolve into amorphous constructions. The technique has proven the exceptional energy of parallel computing utilizing DNA, as it may well resemble an analogue pc and be utilized in selection-based biotechnology strategies, corresponding to DNA-encoded chemical libraries. As a chemical method to unravel issues historically for genetic and statistical evaluation, the strategy offers a fast and cost-efficient analysis of library variety for the intermediate steps by a choice course of.

Consistent variations amongst melting curves of PCR-amplified DNA fragments are handled by normalizing the relative fluorescence models (RFU) and performing a clustering evaluation, however statistically important variations amongst curves usually are not often decided. In the current research, an evaluation primarily based on useful knowledge evaluation (FDA) was applied to judge the existence of statistically important variations between normalized RFU curves obtained from PCR-HRM (high-resolution melting) evaluation by utilizing ANOVA for useful knowledge.

The effectiveness of the FDA technique was analyzed with knowledge from a set of samples of eight animal species of curiosity in meals evaluation, in addition to mixtures of DNA from these species, analyzed by PCR-HRM to distinguish them. The statistical technique described on this research has been demonstrated to be a sturdy and exact device to discriminate amongst melting curves derived from HRM evaluation. This technique has benefits over the present comparability strategies. PRACTICAL APPLICATION: As lengthy as meals fraud and mislabeling exist, new methods for species identification are wanted.

Rapid and Reliable One-Step ABO Genotyping Using Direct Real-Time Allele-Specific PCR and Melting Curve Analysis Without DNA Preparation.

ABO genotyping is a molecular diagnostic method necessary for transfusion and transplantation in medication, and human identification in forensic science. Because ABO genotyping are labor intensive and time consuming, the genotyping can’t be firstly used to resolve the serological ABO discrepancy in blood financial institution. For fast one-step ABO genotyping, we developed direct, real-time, allele-specific polymerase chain response (PCR), and melting curve evaluation (DRAM assay) with out DNA preparation. In DRAM assay, we used a particular PCR buffer for direct PCR, a fast RBC lysis buffer, white blood cells as template with out DNA preparation, allele-specific primers for discriminating three ABO alleles (261G/del, 796C/A, and 803G/C), and melting curve evaluation as a detection technique.

There was 100% concordance among the many outcomes of ABO genotyping by the DRAM assay, serologic typing, PCR-RFLP and PCR-direct sequencing of 96 venous blood samples. We have been capable of cut back the quantity of handbook steps to a few and the hands-on time to 12 min, in comparison with seven steps and roughly 40 min for standard ABO genotyping utilizing allele-specific PCR with purified DNA and agarose gel electrophoresis. We have established and validated the DRAM assay for fast and dependable one-step ABO genotyping in a closed system. The DRAM assay with an acceptable quantity of allele-specific primers may assist in resolving ABO discrepancies and ought to be helpful in scientific laboratory and blood financial institution.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analyses of the genetic variety and inhabitants constructions of Histoplasma capsulatum scientific isolates from Mexico, Guatemala, Colombia and Argentina, utilizing a randomly amplified polymorphic DNAPCR assay.

We studied the genetic variety and the inhabitants construction of human isolates of Histoplasma capsulatum, the causative agent of histoplasmosis, utilizing a randomly amplified polymorphic DNA-polymerase chain response (RAPD-PCR) assay to determine associations with the geographic distribution of isolates from Mexico, Guatemala, Colombia and Argentina. The RAPD-PCR sample analyses revealed the genetic variety by estimating the proportion of polymorphic loci, efficient quantity of alleles, Shannon’s index and heterozygosity. Population construction was recognized by the index of affiliation (IA) take a look at. These knowledge contribute to the information on the molecular epidemiology of histoplasmosis in Latin America.
Thirty-seven isolates have been studied and clustered into three teams by the unweighted pair-group technique with arithmetic imply (UPGMA). Group I contained 5 subgroups primarily based on geographic origin. The consistency of the UPGMA dendrogram was estimated by the cophenetic correlation coefficient (CCCr = 0.94, P = 0.001). Isolates from Mexico and Colombia introduced larger genetic variety than isolates from Argentina. Isolates from Guatemala grouped along with the reference strains from the United States of America and Panama. The IA values recommend the presence of a clonal inhabitants construction within the Argentinian H. capsulatum isolates and additionally validate the presence of recombining populations within the Colombian and Mexican isolates.

Rat Submandibular glandular kallikrein-9 (Klk9) ELISA Kit

abx256273-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

ELISA kit for Rat Submandibular glandular kallikrein-9

EK1729 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Submandibular glandular kallikrein-9 in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

ER0295 96T
EUR 567.6
  • Detection range: 31.25-2000 pg/ml
  • Uniprot ID: P07647
  • Alias: Klk9/klk-9/Tissue kallikrein/Submandibular enzymatic vasoconstrictor(SEV)/KLK-S3/S3 kallikrein
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Mouse Submandibular gland protein C, Muc19 ELISA KIT

ELI-35955m 96 Tests
EUR 865

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m 96 Tests
EUR 865

Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT

ELI-06139r 96 Tests
EUR 886

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 ea Ask for price

Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL

ELI-27901r 96 Tests
EUR 886

Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K

EF017179 96 Tests
EUR 689

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Tumor Tissue: Breast cDNA

HT05-090 10 rxn
EUR 415

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 415

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 415

Human Adult cDNA Tissue: Testis

HA-260 10 rxn
EUR 415

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 445

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 415

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 77.76
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 134
Decoding DNA labels by melting curve analysis using real-time PCR.

Decoding DNA labels by melting curve analysis using real-time PCR.

Synthetic DNA has been used as an authentication code for a various variety of purposes. However, current decoding approaches are primarily based on both DNA sequencing or the willpower of DNA size variations. Here, we current a easy different protocol for labeling totally different objects using a small variety of brief DNA sequences that differ of their melting factors. Code amplification and decoding will be carried out in two steps using quantitative PCR (qPCR). To acquire a DNA barcode with excessive complexity, we outlined Eight template teams, every having four totally different DNA templates, yielding 158 (>2.5 billion) combos of various particular person melting temperature (Tm) values and corresponding ID codes.

The reproducibility and specificity of the decoding was confirmed by using probably the most advanced template combination, which had 32 totally different merchandise in Eight teams with totally different Tm values. The industrial applicability of our protocol was additionally demonstrated by labeling a drone with an oil-based paint containing a predefined DNA code, which was then efficiently decoded. The methodology introduced right here consists of a easy code system primarily based on a small variety of artificial DNA sequences and a cheap, fast decoding protocol using a couple of qPCR reactions, enabling a variety of authentication purposes.

Current research investigated the anti-mosquito potential of Achyranthes aspera towards the dengue vector, Aedes aegypti. The stems and leaves of A. aspera have been extracted in hexane and evaluated for his or her toxicity towards early fourth instars of A. aegypti. The larvicidal efficacy of the extract was validated as per WHO protocol. The mortality counts have been made after 24 h and LC values have been calculated at totally different ranges. No important variations in cfDNA concentrations have been detected between numerous time factors of as much as 24 h till centrifugation.

The antagonistic affect of extracts was additionally explored on the larval genomic DNA. The larvae have been uncovered to extracts at LC50 ranges and the alterations in g-DNA was evaluated via RAPD-PCR method using three random primers; MA-09, MA-12 and MA-26. Our investigations ascertained the larvicidal efficacy of each the leaf and stem extracts of A. aspera leading to respective LC50 values of 0.068 and 0.082 mg/mL. The extracts additionally precipitated variable genotoxic results with important adjustments within the RAPD profiles.

Digital PCR analysis of circulating tumor DNA: a biomarker for chondrosarcoma prognosis, prognostication, and residual illness detection.

Conventional chondrosarcoma is the most typical main bone tumor in adults. Prognosis corresponds with tumor grade however stays variable, particularly for people with grade (G) II illness. There are at the moment no biomarkers out there for monitoring or prognostication of chondrosarcoma. Circulating tumor DNA (ctDNA) has lately emerged as a promising biomarker for a broad vary of tumor sorts. To date, little has been carried out to review the presence of ctDNA and its potential utility within the administration of sarcomas, together with chondrosarcoma.

In this research, we’ve assessed ctDNA ranges in a cohort of 71 sufferers, 32 with sarcoma, together with 29 people with central chondrosarcoma (CS) and 39 with domestically aggressive and benign bone and gentle tissue tumors, using digital PCR. In sufferers with CS, ctDNA was detected in pretreatment samples in 14/29 sufferers, which confirmed clear correlation with tumor grade as demonstrated by the detection of ctDNA in all sufferers with GIII and dedifferentiated illness (n = 6) and in 8/17 sufferers with GII illness, however by no means related to GI CS. Notably detection of ctDNA preoperatively in GII illness was related to a poor final result.

A complete of 14 sufferers with CS had ctDNA ranges assessed at a number of time factors and in most sufferers there was a transparent discount following surgical elimination. This analysis lays the inspiration for bigger research to evaluate the utility of ctDNA for chondrosarcoma prognosis, prognostication, early detection of residual illness and monitoring illness development. DdPCR outcomes on cfDNA are extremely depending on a number of elements throughout preanalytical pattern workup, which have to be addressed through the growth of this diagnostic software for most cancers diagnostics sooner or later.

Decoding DNA labels by melting curve analysis using real-time PCR.

Preanalytical blood pattern workup for cell-free DNA analysis using Droplet Digital PCR for future molecular most cancers diagnostics.

In present molecular most cancers diagnostics, using blood samples of most cancers sufferers for the detection of genetic alterations in plasma (cell-free) circulating tumor DNA (ctDNA) is an rising observe. Since ctDNA ranges in blood are low, extremely delicate Droplet Digital PCR (ddPCR) can be utilized for detecting uncommon mutational targets. In order to carry out ddPCR on blood samples, a standardized process for processing and analyzing blood samples is critical to facilitate implementation into scientific observe. Therefore, we assessed the technical pattern workup process for ddPCR on blood plasma samples.

Genomic DNA from Human Adult Normal Tissue: Uterus, from a single donor

D1234274 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Tissue: Uterus Tumor, from a single donor

D1235274 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Kit

20-abx098076
  • EUR 439.00
  • EUR 244.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA

X11000 0.2 ml Ask for price
Description: reagents widely cited

Genomic DNA from Human Adult Normal Tissue: Uterus: Cervix, from a single donor

D1234275 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA - Rat Male

D1434999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female

D1434999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male

D1734999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male

D1834999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male

D1934999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male

D1B34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male

D1C34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male

D1H34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male

D1O34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Male

D1234999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA Kit

20-abx098077
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Micro Genomic DNA Kit

20-abx098242
  • EUR 105.00
  • EUR 328.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 272
  • Shipped within 5-10 working days.

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 398
  • Shipped within 5-12 working days.

Animal Genomic DNA Kit

abx294005-100preps 100 preps
EUR 523
  • Shipped within 5-12 working days.

Animal Genomic DNA Kit

abx294005-50preps 50 preps
EUR 398
  • Shipped within 5-12 working days.

Genomic DNA Extraction Kit

K5016005 1 kit
EUR 329

Genomic DNA Isolation Kit

K281-50
EUR 272

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 321

Soil Genomic DNA Kit

K1411-250
EUR 561

Soil Genomic DNA Kit

K1411-50
EUR 316

Insect Genomic DNA Kit

K1412-250
EUR 561

Insect Genomic DNA Kit

K1412-50
EUR 316

Mollusc Genomic DNA Kit

K1413-250
EUR 561

Mollusc Genomic DNA Kit

K1413-50
EUR 316

Yeast Genomic DNA Kit

K1414-250
EUR 561

Yeast Genomic DNA Kit

K1414-50
EUR 316

Fungal Genomic DNA Kit

K1415-250
EUR 561

Fungal Genomic DNA Kit

K1415-50
EUR 316

Human Brain Genomic DNA

X11001 10 µg Ask for price
Description: kits suitable for this type of research

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male

D1534999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male

D1G34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA extraction buffer

20-abx098075
  • EUR 286.00
  • EUR 105.00
  • 100 ml
  • 1.2 ml
  • Shipped within 5-10 working days.

Marine Animal Genomic DNA Kit

20-abx098079
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

FFPE Tissue Genomic DNA Kit

abx098866-50rxns 50 rxns
EUR 272
  • Shipped within 5-10 working days.

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P 50 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P 25 Preps Ask for price

AccuRT Genomic DNA Removal Kit

G488 200 Reactions
EUR 82

Genomic DNA Extraction and Purification

EP10012 100 Tests
EUR 505

Bacterial Genomic DNA Isolation Kit

K309-100
EUR 376

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 550

Genomic DNA from Mouse Normal Tissue: Brain

D1334035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Heart

D1334122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Liver

D1334149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Lung

D1334152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Spleen

D1334246 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Stomach

D1334248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 250 prep
EUR 536

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Genomic DNA Kit (No RNase A)

20-abx098862
  • EUR 398.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Mammalian Cell Genomic DNA Isolation Kit

K967-100
EUR 381

Plant Tissue Genomic DNA Isolation Kit

K316-100
EUR 376

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price
Description: The best epigenetics products

Rapid Animal Genomic DNA Extraction Kit

AT4780 10Preps
EUR 69.03
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit

AT4781 50Preps
EUR 126.13
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit

AT4782 250preps
EUR 354.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

Rapid Yeast Genomic DNA Extraction Kit

BS8227 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

Rapid Blood Genomic DNA Extraction Kit

BT4782 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

Genomic DNA from Mouse Normal Tissue: Skeletal Muscle

D1334171 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

LumiPure genomic DNA from AnySample Kit, 50

21573 50 preps
EUR 108

Genomic DNA from Rat Normal Tissue: Brain

D1434035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Blood samples from wholesome people, in addition to lung most cancers sufferers have been analyzed. We in contrast totally different strategies and protocols for pattern assortment, storage, centrifugation, isolation, and quantification. Cell-free DNA (cfDNA) concentrations of a number of wild-type targets and BRAF and EGFR-mutant ctDNA concentrations quantified by ddPCR have been main final result measurements. Highest cfDNA concentrations have been measured in blood collected in serum tubes.  Highest cfDNA concentrations have been detected after DNA isolation with the Quick cfDNA Serum & Plasma Kit, whereas plasma isolation using the QIAamp Circulating Nucleic Acid Kit yielded probably the most constant outcomes.

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Major histocompatibility complicated (MHC) represents an essential genetic marker for manipulation to enhance the well being and productiveness of cattle. It is intently related to quite a few illness susceptibilities and immune responses. Bovine MHC, additionally known as bovine leukocyte antigen (BoLA), is taken into account as an acceptable marker for genetic variety research. In cattle, most of the polymorphisms are positioned in exon 2 of BoLA-DRB3, which encodes the peptide-binding cleft. In this research, the polymorphism of the BoLA-DRB3.2 gene in Holstein’s calves was studied utilizing excessive decision melting curve analysis (HRM).

Observed HRM outcomes have been in comparison with PCR-RFLP and direct sequencing strategies. Eight completely different HRM and seven completely different RFLP profiles have been recognized among the many inhabitants studied. By evaluating to sequencing knowledge, HRM may fully discriminate all genotypes (eight profiles), whereas the RFLP failed to differentiate between the genotypes *1101/*1001 and *1104/*1501. According to the outcomes, the HRM analysis methodology gave extra correct outcomes than RFLP by differentiating between the BoLA-DRB3.2 genotypes.

Due to the Co-dominant nature of the MHC alleles, HRM method might be used for investigating the polymorphisms of genotypes and their associations with immune responses. In transfection experiments with mammalian cells aiming to overexpress a selected protein, it’s usually essential to appropriately quantify the extent of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts have been transfected with a vector containing the whole Pex11β cDNA (plasmid DNA).

The Pex11β mRNA stage, as calculated utilizing the RT-qPCR product, was unrealistically larger (>1000-fold) in transfected in comparison with non-transfected cells, and we assumed that there have been giant quantities of contaminating plasmid DNA within the RNA pattern. Thus, we looked for a easy method to distinguish between plasmid-derived mRNA, endogenous genome-derived mRNA and plasmid DNA, with minimal adjustments to straightforward RT-PCR strategies.

Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis.

Epidermal development issue receptor (EGFR) mutation testing in plasma cell-free DNA (cfDNA) from superior lung most cancers sufferers is an rising scientific software. This meta-analysis was designed to find out the diagnostic accuracy of two widespread PCR programs, droplet digital PCR (ddPCR) and amplification refractory mutation system PCR (ARMS-PCR), for detecting EGFR mutation in cfDNA. A scientific search was carried out primarily based on PubMed, Web of science, Embase and the Cochrane library. Data from eligible research have been extracted and pooled to calculate the sensitivity, specificity, diagnostic odds ratio (DOR), space below the abstract receiver-operating attribute curve (AUROC), utilizing tissue biopsy outcomes as the usual methodology.

Subgroup analyses have been carried out relating to EGFR mutation kind, tumor stage, and EGFR-TKI remedy. Twenty-five research involving 4,881 circumstances have been included. The plasma testing sensitivity, specificity, DOR, and AUROC, in contrast with the matched tumor tissues, have been 72.1%, 95.6%, 38.5, 0.89 for ddPCR, and 65.3%, 98.2%, 52.8, 0.71 for ARMS-PCR, respectively, by oblique comparability, vital variations have been present in sensitivity (P = 0.003) and specificity (P = 0.007).

Furthermore, vital distinction was present in sensitivity between tumor stage subgroups (IIIB-IV subgroup vs. IA-IV subgroup) in ARMS-PCR (73.7 vs. 64.2%, P = 0.008), however not in ddPCR (72.5 vs. 71.2%, P = 0.756). This research demonstrates that ddPCR and ARMS-PCR have a excessive specificity with a sensible sensitivity for detecting EGFR mutation in cfDNA, which helps their utility as a complement or a conditional-alternative to tissue biopsy in scientific follow for genotyping. It appears that ddPCR has the next sensitivity than ARMS-PCR, particularly in early phases.

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity analysis and complementing forensic surveillance.

Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is important for the prosecution of illegally-traded wildlife merchandise, conservation-based biodiversity analysis, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue depends on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which stays pricey for functions of screening giant numbers of unknown samples throughout routine surveillance.

Here, we tailored a fast, low-cost strategy to distinguish 10 home and 24 wildlife species which might be widespread within the East African unlawful wildlife merchandise commerce primarily based on their distinctive high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR merchandise. Using the strategy, we recognized (i) giraffe amongst covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences amongst savannah elephant reference samples. This strategy is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.

Universal DNA Marker (100 bp ~ 10,000 bp)

W2583-50 NULL
EUR 0

Universal DNA Marker (100 bp ~ 10,000 bp)

W2583-500 NULL
EUR 0

Methylamp Universal Methylated DNA Kit

P-1011 20 Modifications
EUR 570.55
Description: kits suitable for this type of research

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 347.25
Description: The best epigenetics products

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-01 24 rxn
EUR 733

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-02 96 rxn
EUR 2136

100bp DNA Marker

D1100-050 50㎍
EUR 165

100bp DNA Marker

D1100-250 5X50㎍
EUR 550

1Kb DNA Marker

D1101-050 50㎍
EUR 131

1Kb DNA Marker

D1101-250 5X50㎍
EUR 300

2K DNA Marker

20-abx098045
  • EUR 258.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

5K DNA Marker

20-abx098048
  • EUR 258.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

15K DNA Marker

20-abx098049
  • EUR 272.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

1Kb DNA Marker

20-abx098050
  • EUR 258.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

100bp DNA Marker

20-abx098052
  • EUR 272.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

DL5000 DNA Marker

MD102-01 250 μl
EUR 107

DL5000 DNA Marker

MD102-02 500 μl
EUR 113

DL15000 DNA Marker

MD103-01 250 μl
EUR 109

DL15000 DNA Marker

MD103-02 500 μl
EUR 115

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-01 24 rxn
EUR 441

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-02 96 rxn
EUR 1405

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-01 24 rxn
EUR 465

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-02 96 rxn
EUR 1549

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-01 24 rxn
EUR 492

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-02 96 rxn
EUR 1605

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-01 24 rxn
EUR 733

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-02 96 rxn
EUR 2136

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-01 24 rxn
EUR 756

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-02 96 rxn
EUR 2284

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-01 24 rxn
EUR 824

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-02 96 rxn
EUR 2408

UNIVERSAL PLATFORM

480144 1/pk
EUR 269
Description: Lab Equipment; Constant Temperature Equipment

1Kb Plus DNA Marker

20-abx098051
  • EUR 300.00
  • EUR 203.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

DL2000 Plus DNA Marker

MD101-01 250 μl
EUR 107

DL2000 Plus DNA Marker

MD101-02 500 μl
EUR 113

Linear Kinetoplast DNA Marker

TG2018-1 10 ug
EUR 179

Decatenated Kinetoplast DNA Marker

TG2020-1 10 ug
EUR 190

Relaxed pRYG DNA Marker

TG2025-1 10 ug
EUR 268

Linear pRYG DNA Marker

TG2028-1 10 ug
EUR 179

1 Kb Ladder DNA Marker

FYD001-500UL 20 µg/ 500 µl Ask for price

100 bp Ladder DNA Marker

FYD002-500UL 30 µg/ 500 µl Ask for price

50 bp Ladder DNA Marker

FYD004-500UL 32.5 µg/ 500 µl Ask for price

Universal Coating Solution

AC002 20 ml
EUR 297

Universal Detachment Solution

AD002 100 ml
EUR 148

Universal Spring Platform

H1000-P-SP 1 PC
EUR 323.39
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Universal Neutralization Buffer

NB001 100 ml
EUR 135

UNIVERSAL BEER AGAR

U21-100-10kg 10 kg
EUR 1524

UNIVERSAL BEER AGAR

U21-100-2kg 2kg
EUR 370

UNIVERSAL BEER AGAR

U21-100-500g 500 g
EUR 137

UNIVERSAL PREENRICHMENT BROTH

U21-101-10kg 10 kg
EUR 749

UNIVERSAL PREENRICHMENT BROTH

U21-101-2Kg 2 Kg
EUR 202

UNIVERSAL PREENRICHMENT BROTH

U21-101-500g 500 g
EUR 91

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-03 24 rxn
EUR 429

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-04 96 rxn
EUR 1354

Image Green? 100 bp DNA Marker

M1155-500
EUR 294

Image Green? 1 kb DNA Marker

M1156-500
EUR 294

50-500bp Low Range DNA Marker

GM305 200loading, 200prep
EUR 114.82
  • Product category: Electrophoresis Related/Ladders - DNA/50-500 bp

500-10000bp DNA Marker, Original Form

M101O-1 100loading, 100prep
EUR 91.76
  • Product category: Electrophoresis Related/Ladders - DNA/500-10000 bp

500-10000bp DNA Marker, Original Form

M101O-2 5x100loading, 500prep
EUR 219.65
  • Product category: Electrophoresis Related/Ladders - DNA/500-10000 bp

100-1500bp DNA Marker, Original Form

M102O-1 100loading, 100prep
EUR 90.02
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

100-1500bp DNA Marker, Original Form

M102O-2 5x100loading, 500prep
EUR 215.3
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

100-1500bp DNA Marker, Original Form

M107O-1 100loading, 100prep
EUR 91.76
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

100-1500bp DNA Marker, Original Form

M107O-2 5x100loading, 500prep
EUR 219.65
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

2K Plus DNA Marker (8 linear double-stranded DNA bands)

20-abx098046
  • EUR 258.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

2K Plus DNA Marker (9 linear double-stranded DNA bands)

20-abx098047
  • EUR 258.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

100bp Plus DNA Marker (12 linear double-stranded DNA bands)

20-abx098053
  • EUR 272.00
  • EUR 189.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

100bp Plus DNA Marker (14 linear double-stranded DNA bands)

20-abx098054
  • EUR 300.00
  • EUR 203.00
  • 2.5 ml
  • 500 ul
  • Shipped within 5-10 working days.

Universal Casein Diluent/Blocker

85R-108 250 ml
EUR 230
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates

5min Universal Ligation Mix

C311-01 50 rxn
EUR 214

5min Universal Ligation Mix

C311-02 100 rxn
EUR 283

PCR plate-foil universal

A28216 1x100 pcs
EUR 94

PCR plate-foil universal

A28216L 5x100 pcs
EUR 310

Universal Full Growth Medium

TM001 500 ml
EUR 336

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 81.32
  • Product category: Molecular Biology Kits/Protein - Purification/Precipitation

Tumour Marker MUC1 (S1.1) , DNA Aptamer, Biotinylated

AD-152-B Custom Ask for price

Tumour Marker MUC1 (S1.1) , DNA Aptamer, unlabeled

AD-152-U Custom Ask for price

100-1200bp DNA Marker, Ready-to-use

GM333 50loading, 50prep
EUR 67.4
  • Product category: Electrophoresis Related/Ladders - DNA/100-1200 bp

100-1200bp DNA Marker, Ready-to-use

GM334 5x50loading, 250prep
EUR 128.3
  • Product category: Electrophoresis Related/Ladders - DNA/100-1200 bp

100-2000bp DNA Marker, Ready-to-use

GM335 50loading, 50prep
EUR 67.4
  • Product category: Electrophoresis Related/Ladders - DNA/100-2000 bp

100-2000bp DNA Marker, Ready-to-use

GM336 5x50loading, 250prep
EUR 128.3
  • Product category: Electrophoresis Related/Ladders - DNA/100-2000 bp

200-1500bp DNA Marker, Ready-to-use

GM337 50loading, 50prep
EUR 67.4
  • Product category: Electrophoresis Related/Ladders - DNA/200-1500 bp

200-1500bp DNA Marker, Ready-to-use

GM338 5x50loading, 250prep
EUR 128.3
  • Product category: Electrophoresis Related/Ladders - DNA/200-1500 bp

200-2000bp DNA Marker, Ready-to-use

GM339 50loading, 50prep
EUR 67.4
  • Product category: Electrophoresis Related/Ladders - DNA/200-2000 bp

200-2000bp DNA Marker, Ready-to-use

GM340 5x50loading, 250prep
EUR 128.3
  • Product category: Electrophoresis Related/Ladders - DNA/200-2000 bp

300-2500bp DNA Marker, Ready-to-use

GM341 50loading, 50prep
EUR 67.4
  • Product category: Electrophoresis Related/Ladders - DNA/300-2500 bp

300-2500bp DNA Marker, Ready-to-use

GM342 5x50loading, 250prep
EUR 128.3
  • Product category: Electrophoresis Related/Ladders - DNA/300-2500 bp

100-1000bp DNA Marker, Ready-to-use

GM343 50loading, 50prep
EUR 80.45
  • Product category: Electrophoresis Related/Ladders - DNA/100-1000 bp

100-1000bp DNA Marker, Ready-to-use

GM345 50loading, 50prep
EUR 84.8
  • Product category: Electrophoresis Related/Ladders - DNA/100-1000 bp

500-10000bp DNA Marker, Ready-to-use

M101R-1 100loading, 100prep
EUR 91.76
  • Product category: Electrophoresis Related/Ladders - DNA/500-10000 bp

500-10000bp DNA Marker, Ready-to-use

M101R-2 5x100loading, 500prep
EUR 219.65
  • Product category: Electrophoresis Related/Ladders - DNA/500-10000 bp

100-1500bp DNA Marker, Ready-to-use

M102R-1 100loading, 100prep
EUR 90.02
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

100-1500bp DNA Marker, Ready-to-use

M102R-2 5x100loading, 500prep
EUR 215.3
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

Lambda DNA/HindIII Marker, Ready-to-use

M104R-1 100loading, 100prep
EUR 65.66
  • Product category: Electrophoresis Related/Ladders - DNA/Lambda DNA/HindIII

Lambda DNA/HindIII Marker, Ready-to-use

M104R-2 5x100loading, 500prep
EUR 124.82
  • Product category: Electrophoresis Related/Ladders - DNA/Lambda DNA/HindIII

100-1500bp DNA Marker, Ready-to-use

M107R-1 100loading, 100prep
EUR 91.76
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

100-1500bp DNA Marker, Ready-to-use

M107R-2 5x100loading, 500prep
EUR 219.65
  • Product category: Electrophoresis Related/Ladders - DNA/100-1500 bp

The mouse transplantation mannequin stays essentially the most related methodology to evaluate the practical capacities of mammary cells and is especially applicable for investigations relating to mammary stem cells, regardless of the species studied. Following xenotransplantation in mice mammary fats pad, the event of the xenograft is usually evaluated by immunohistology. Here, we current a easy and fast methodology to manage the species specificity of a xenograft primarily based on genomic DNA PCR amplification. DNA is extracted from the mounted samples meant for histology, thus permitting the reuse of treasured samples. Standard and digital droplet PCR (requiring low DNA portions) strategies have been used to make the current methodology appropriate for the analysis of xenotransplanted samples.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

Overwhelming proof means that in addition to the genetic adjustments of DNA mutations, epigenetic adjustments of DNA methylation and histone modifications play vital position in regulation of gene expression. DNA methylation is essentially the most frequent epigenetic alteration noticed in mammalian genomes, and usually it’s negatively correlated with gene expression. Various strategies can be found for the detection of DNA methylation adjustments. Although the current high-throughput strategies for DNA methylation evaluation have varied benefits, they require excessive ranges of technical experience, pricey gear, and reagents.

Because of these causes, many of the worldwide DNA methylation evaluation strategies are primarily carried out at core facility, and laboratories with restricted assets and experience are usually not ready to make use of these strategies. Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) is a restriction enzyme digestion and PCR-based technique for the evaluation of DNA methylation adjustments.

This technique is cost-effective, requires easy and fundamental instrumentation, and due to this fact can simply be carried out in any laboratory with fundamental setup having an everyday DNA thermal cycler and DNA gel electrophoresis system. Additional benefits of this technique over different strategies for DNA methylation evaluation are that it requires very much less quantity of DNA and might display DNA methylation adjustments globally at genome-wide stage with excessive sensitivity. This technique has been efficiently used to detect adjustments in DNA methylation both occurring naturally or induced by varied toxicants and environmental components. A element experimental protocol for MS-RAPD-PCR is described in this chapter.

Laphet is a standard fermented meals in Myanmar, constructed from tea leaves (Camellia sinensis) by fermentation with restricted air passage. We carried out microbial range analyses on 14 Laphet merchandise collected from totally different places in Myanmar. Amplicon-based sequencing outcomes revealed Lactobacillus and Acetobacter had been plentiful micro organism and Candida, Pichia, Cyberlindnera, and Debaryomyces had been plentiful yeast. Using selective media, eight species of lactic acid micro organism and 9 species of yeast had been remoted; Lactobacillus plantarum and L. collinoides had been dominant micro organism and Pichia manshurica, Candida boidinii, and Cyberlindnera jadinii had been main yeasts. PCR-DGGE evaluation confirmed that almost all of the dominant bacterial and yeast species discovered in tradition dependent evaluation had been current in Laphet samples.

High density DNA methylation array is a dependable various for PCR-based evaluation of the MGMT promoter methylation standing in glioblastoma.

MGMT promoter methylation standing is a crucial biomarker predicting survival and response to chemotherapy in sufferers affected by glioblastoma. Since new diagnostic strategies akin to methylome-based classification of mind tumors are increasingly continuously carried out, we geared toward evaluating the suitability of calculating the MGMT promoter methylation standing in a quantitative method from the methylome profiling as in comparison with the traditional gold normal evaluation by PCR.Our cohort consisted of 39 instances identified as “glioblastoma, IDH-wildtype” of which the MGMT promoter methylation standing was analyzed with each methylation-specific PCR and excessive density DNA methylation array utilizing the STP-27 algorithm.

Contradictory outcomes had been validated by pyrosequencing.The inter-method reliability reached 77% (kappa-coefficient: 0.58) when additionally instances with an inconclusive end result in one or the opposite technique had been taken into consideration. When solely instances with conclusive outcomes in each strategies had been thought of, a really excessive inter-method reliability of 91% (kappa-coefficient: 0.86) could possibly be achieved. For “methylated” instances, no contradictory outcomes had been obtained.

For the remaining two instances with discrepant outcomes subsequent pyrosequencing analyses spoke in favor of every beforehand utilized technique as soon as.In addition to its advantages for molecular subgrouping and duplicate quantity evaluation of mind tumors, DNA-methylation based mostly classification is a extremely dependable instrument for the evaluation of MGMT promoter methylation standing in glioblastoma sufferers.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis.

Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been a staple of analysis, offering valuable assets for molecular and genomic research. However, the most important problem is the extraction of high-quality DNA from FFPE tissues, on condition that the integrity of DNA is critically affected by formalin fixation. Formaldehyde induces crosslinks in DNA that renders single or double-stranded DNA breaks. Such breaks trigger in depth fragmentation that instantly influences the standard of DNA purified and the quantity of templates out there for PCR amplification. Thus, protocol for DNA purification from FFPE tissues should successfully extract extremely fragmented DNA and reverse cross-linking brought about by formalin fixation.

DNA extraction strategies out there in the literature had been chosen and modified at totally different phases to optimize a protocol that extracts DNA of adequate high quality and fragment dimension to be detectable by PCR. Archived FFPE tissues belonged to sufferers with triple damaging breast most cancers (TNBC) and benign breast illness had been used for the protocol optimization. The finest optimized protocol was then used to amplify Exon four area of Proviral integration web site for Moloney murine leukemia virus1 (Pim1) kinase gene to research any possible somatic mutations each in TNBCs and benign breast illnesses.

cDNA from Dog Normal Tissue: Kidney

C1734142 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Kidney

C1236142Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Matching Pair - cDNA from Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 499
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Cynomolgus) cDNA Normal Tissue: Liver

MC34-149 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Spleen

MC34-246 10 rxn
EUR 415

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adipose

C1534003-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Appendix

C1534006-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Bladder

C1534010 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Bladder

C1534010-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Brain

C1534035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Brain

C1534035-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Brain:Cerebellum

C1534039-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Cecum

C1534089 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Of the 12 totally different protocols developed, highest quality DNA in phrases of fragment dimension and purity was obtained when Tween20 lysis buffer was used for each deparaffinization and in a single day digestion together with excessive salt precipitation. Optimized protocol was then validated by extracting DNAs from 10 TNBCs and 5 benign breast illness specimens with constant purity and fragment dimension. PCR amplification and subsequent Sanger’s sequencing revealed the presence of mutations in the Exon four area of Pim1 kinase. Deparaffinization and in a single day digestion in Tween20 lysis buffer together with excessive salt precipitation yielded the highest quality PCR amplifiable DNA for mutational evaluation.

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

Polymerase chain response (PCR) using deoxyribonucleic acid (DNA) extracted from dried blood spots (DBS) supplies a quick, cheap, and handy methodology for large-scale epidemiological research. This research in contrast the performance of PCR between DNA extracted from DBS and DNA obtained from whole blood for detecting malarial parasites. Primary research assessing the diagnostic performance of PCR using DNA extracted from DBS and whole blood for detecting malarial parasites have been obtained from the ISI Web of Science, Scopus, and PubMed databases. Odds ratios (ORs) and 95% confidence intervals (CIs) have been plotted in forest plots using Review Manager model 5.3. Statistical evaluation was carried out through random-effects meta-analysis.

Data heterogeneity was assessed using the I2 statistic. Of the 904 research retrieved from the databases, seven have been included on this research. The pooled meta-analysis demonstrated no vital distinction within the comparative performance of PCR for detecting malaria parasites between DNA extracted from DBS and that extracted from whole blood (OR 0.85; 95% CI 0.62-1.16; I2 = 78%). However, subgroup evaluation demonstrated that PCR using DNA extracted from DBS was much less correct in detecting Plasmodium vivax than that using DNA extracted from whole blood (OR = 0.85; 95% CI 0.77-0.94).

In conclusion, a vital distinction in detecting P. vivax was noticed between PCR using DNA extracted from DBS and that using DNA extracted from whole blood. Therefore, P. vivax in endemic areas ought to be recognized and detected with care with PCR using DNA obtained from DBS which doubtlessly results in a unfavourable outcome. Further research are required to research the performance of PCR using DBS for detecting P. vivax and different malarial parasites to supply knowledge in analysis and routine surveillance of malaria, particularly with renewed efforts in the direction of the eradication of the illness.

It is commonly troublesome to tell apart morphologically between intently associated species of fleas (Siphonaptera). Morphological identification of fleas usually requires microscopic examination of inner buildings in specimens cleared using caustic options. This course of degrades DNA and/or inhibits DNA extraction from specimens, which limits molecular-based research on particular person fleas and their microbiomes. Our goal was to tell apart between Oropsylla rupestris (Jordan), Oropsylla tuberculata (Baker), Oropsylla bruneri (Baker), and Oropsylla labis (Jordan & Rothschild) (Ceratophyllidae) using PCR-based single strand conformation polymorphism (SSCP) analyses and DNA sequencing. A 446 bp area of the nuclear 28S ribosomal RNA (rRNA) gene was used because the genetic marker.

Ionizing radiation, genotoxic stress, and mitochondrial DNA copy-number variation in Caenorhabditis elegans: droplet digital PCR evaluation

Mitochondria are weak to the results of ionizing radiation; harm to mitochondrial DNA (mtDNA) could also be extra intensive and persistent than harm to nuclear DNA (nDNA). Variation in mtDNA copy quantity has been proposed as a marker for mitochondrial dysfunction in response to ionizing radiation. We have developed a exact and delicate duplex droplet digital PCR (ddPCR) methodology for quantitation of the mtDNA/nDNA ratio within the mannequin organism Caenorhabditis elegans. The impact on this ratio was investigated over a big selection of doses (0.03-72 Gy) of power gamma irradiation.

Five mitochondrial targets and two nuclear reference genes have been amplified pairwise in duplex PCR format (one mitochondrial and one nuclear goal per PCR) by each ddPCR and quantitative PCR (qPCR). The outcomes confirmed that ddPCR however not qPCR enabled detection of a vital enhance in mtDNA copy quantity (1.6 ± 0.1-fold) for nematodes uncovered to excessive doses (≥24 Gy). Thus, ddPCR offered larger precision and larger sensitivity than qPCR for detection of mtDNA copy quantity variation. The variation adopted a Hill-type dose response with threshold 10.3 ± 1 Gy. This strongly means that power genotoxic stress impacts mtDNA replication. The duplex ddPCR methodology is a novel, high-precision, delicate software for willpower of mitochondrial DNA copy quantity variation and operate in C. elegans.

The outcomes obtained for 36 reference specimens (i.e., fleas that have been morphologically recognized to species) revealed no intraspecific variation in DNA sequence, whereas the DNA sequences of the 4 species of Oropsylla differed from each other at two to 6 nucleotide positions. Each flea species additionally had a distinctive SSCP banding sample. SSCP analyses have been then used to establish one other 84 fleas that had not been recognized morphologically. DNA sequencing knowledge confirmed the species id of fleas subjected to SSCP. This demonstrates that PCR-SSCP mixed with DNA sequencing of the 28S rRNA gene is a very efficient method for the delineation of 4 intently associated species of flea.

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

The diagnostic accuracy of digital PCR, ARMS and NGS for detecting KRAS mutation in cell-free DNA of sufferers with colorectal most cancers: A protocol for systematic assessment and meta-evaluation

Cetuximab and panitumumab have been used clinically to deal with metastatic colorectal most cancers for greater than 15 years. Before the remedy is given, it’s required to find out the KRAS mutation standing since it might result in drug resistance. Tumor tissue pattern is historically used for most cancers genotyping. In current years, liquid biopsy pattern has been intensively investigated as a surrogate for tumor tissue pattern resulting from its non-invasiveness and higher presentation of tumor heterogeneity.

Anti-DNAM-1 antibody

STJ96767 200 µl
EUR 197
Description: Rabbit polyclonal to DNAM-1.

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Anti-Phospho-DNAM-1 (S329) antibody

STJ91074 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-DNAM-1 (S329).

CD226/DNAM-1 Antibody

AF4778 200ul
EUR 376
Description: CD226/DNAM-1 Antibody detects endogenous levels of CD226/DNAM-1.

CD226/DNAM-1 Antibody

AF0087 200ul
EUR 304
Description: CD226/DNAM-1 antibody detects endogenous levels of total CD226/DNAM-1.

DNAM-1 Polyclonal Antibody

ES4132-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4132-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

CD226 / DNAM-1 Antibody

20-abx119383