Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

The research of populations of massive dimension and excessive variety is proscribed by the aptitude of gathering knowledge. Moreover, for a pool of people, every related to a distinctive attribute function, because the pool dimension grows, the potential interactions enhance exponentially, rapidly past the restrict of computation and experimental research. Herein, we current designs of DNA libraries with numerous variety. Using a facile analytic technique primarily based on actual time PCR, we are able to consider the range of a pool of DNA permitting terribly excessive heterogenicity (e.g. > 1 trillion).

We demonstrated that these DNA libraries can be utilized to mannequin heterogeneous populations, exhibiting capabilities corresponding to self-protection, appropriate for biased enlargement, and to evolve into amorphous constructions. The technique has proven the exceptional energy of parallel computing utilizing DNA, as it may well resemble an analogue pc and be utilized in selection-based biotechnology strategies, corresponding to DNA-encoded chemical libraries. As a chemical method to unravel issues historically for genetic and statistical evaluation, the strategy offers a fast and cost-efficient analysis of library variety for the intermediate steps by a choice course of.

Consistent variations amongst melting curves of PCR-amplified DNA fragments are handled by normalizing the relative fluorescence models (RFU) and performing a clustering evaluation, however statistically important variations amongst curves usually are not often decided. In the current research, an evaluation primarily based on useful knowledge evaluation (FDA) was applied to judge the existence of statistically important variations between normalized RFU curves obtained from PCR-HRM (high-resolution melting) evaluation by utilizing ANOVA for useful knowledge.

The effectiveness of the FDA technique was analyzed with knowledge from a set of samples of eight animal species of curiosity in meals evaluation, in addition to mixtures of DNA from these species, analyzed by PCR-HRM to distinguish them. The statistical technique described on this research has been demonstrated to be a sturdy and exact device to discriminate amongst melting curves derived from HRM evaluation. This technique has benefits over the present comparability strategies. PRACTICAL APPLICATION: As lengthy as meals fraud and mislabeling exist, new methods for species identification are wanted.

Rapid and Reliable One-Step ABO Genotyping Using Direct Real-Time Allele-Specific PCR and Melting Curve Analysis Without DNA Preparation.

ABO genotyping is a molecular diagnostic method necessary for transfusion and transplantation in medication, and human identification in forensic science. Because ABO genotyping are labor intensive and time consuming, the genotyping can’t be firstly used to resolve the serological ABO discrepancy in blood financial institution. For fast one-step ABO genotyping, we developed direct, real-time, allele-specific polymerase chain response (PCR), and melting curve evaluation (DRAM assay) with out DNA preparation. In DRAM assay, we used a particular PCR buffer for direct PCR, a fast RBC lysis buffer, white blood cells as template with out DNA preparation, allele-specific primers for discriminating three ABO alleles (261G/del, 796C/A, and 803G/C), and melting curve evaluation as a detection technique.

There was 100% concordance among the many outcomes of ABO genotyping by the DRAM assay, serologic typing, PCR-RFLP and PCR-direct sequencing of 96 venous blood samples. We have been capable of cut back the quantity of handbook steps to a few and the hands-on time to 12 min, in comparison with seven steps and roughly 40 min for standard ABO genotyping utilizing allele-specific PCR with purified DNA and agarose gel electrophoresis. We have established and validated the DRAM assay for fast and dependable one-step ABO genotyping in a closed system. The DRAM assay with an acceptable quantity of allele-specific primers may assist in resolving ABO discrepancies and ought to be helpful in scientific laboratory and blood financial institution.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analyses of the genetic variety and inhabitants constructions of Histoplasma capsulatum scientific isolates from Mexico, Guatemala, Colombia and Argentina, utilizing a randomly amplified polymorphic DNAPCR assay.

We studied the genetic variety and the inhabitants construction of human isolates of Histoplasma capsulatum, the causative agent of histoplasmosis, utilizing a randomly amplified polymorphic DNA-polymerase chain response (RAPD-PCR) assay to determine associations with the geographic distribution of isolates from Mexico, Guatemala, Colombia and Argentina. The RAPD-PCR sample analyses revealed the genetic variety by estimating the proportion of polymorphic loci, efficient quantity of alleles, Shannon’s index and heterozygosity. Population construction was recognized by the index of affiliation (IA) take a look at. These knowledge contribute to the information on the molecular epidemiology of histoplasmosis in Latin America.
Thirty-seven isolates have been studied and clustered into three teams by the unweighted pair-group technique with arithmetic imply (UPGMA). Group I contained 5 subgroups primarily based on geographic origin. The consistency of the UPGMA dendrogram was estimated by the cophenetic correlation coefficient (CCCr = 0.94, P = 0.001). Isolates from Mexico and Colombia introduced larger genetic variety than isolates from Argentina. Isolates from Guatemala grouped along with the reference strains from the United States of America and Panama. The IA values recommend the presence of a clonal inhabitants construction within the Argentinian H. capsulatum isolates and additionally validate the presence of recombining populations within the Colombian and Mexican isolates.

Mouse C57 Salivary gland, Submandibular cDNA-Random Primer

MD-317-C57-RH 30 reactions
EUR 280

Mouse CD1 Salivary gland, Submandibular cDNA-Random Primer

MD-317-HR 30 reactions
EUR 243

Glandular kallikrein-3, submandibular

AP84287 1mg
EUR 2640

Submandibular glandular kallikrein-9

AP84804 1mg
EUR 2640

Submandibular Gland Renin antibody (Mouse)

10R-S119a 200 ul
EUR 548.4
Description: Mouse monoclonal Submandibular Gland Renin antibody (Mouse)

Immortalized Human Submandibular Gland Cells

T0732 1x10^6 cells / 1.0 ml
EUR 2750

Glandular kallikrein-7, submandibular/renal

AP84634 1mg
EUR 2640

Muc10 (untagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369), (10ug)

MC218203 10 µg Ask for price

Muc10 (untagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946), (10ug)

MC206844 10 µg Ask for price

Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946)

MG201463 10 µg Ask for price

Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369)

MG203492 10 µg Ask for price

Mouse Submandibular gland protein C, Muc19 ELISA KIT

ELI-35955m 96 Tests
EUR 1038

Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946)

MR201463 10 µg Ask for price

Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369)

MR203492 10 µg Ask for price

Rat Glandular kallikrein-7, submandibular/renal (Klk7)

1-CSB-EP012458RA
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat Glandular kallikrein-7, submandibular/renal(Klk7) expressed in E.coli

ELISA kit for Rat Submandibular glandular kallikrein-9

EK1729 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Submandibular glandular kallikrein-9 in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Submandibular glandular kallikrein-9 (Klk9) ELISA Kit

abx256273-96tests 96 tests
EUR 801.6

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

ER0295 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Klk9/ Submandibular glandular kallikrein-9 ELISA Kit

E0548Ra 1 Kit
EUR 685.2

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

EKF58847-48T 48T
EUR 396.9

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

EKF58847-5x96T 5x96T
EUR 2693.25

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

EKF58847-96T 96T
EUR 567

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m 96 Tests
EUR 1038

Smgc (untagged) - Mouse submandibular gland protein C (Smgc), (10ug)

MC221197 10 µg Ask for price

Smgc (untagged ORF) - Rat submandibular gland protein C (Smgc), (10 ug)

RN212008 10 µg Ask for price

Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL

ELI-27901r 96 Tests
EUR 1063.2

Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT

ELI-06139r 96 Tests
EUR 1063.2

Smgc (GFP-tagged) - Mouse submandibular gland protein C (Smgc), (10ug)

MG219211 10 µg Ask for price

Lenti ORF clone of Muc10 (mGFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946)

MR201463L4 10 µg Ask for price

Lenti ORF clone of Muc10 (mGFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369)

MR203492L4 10 µg Ask for price

Smgc (Myc-DDK-tagged) - Mouse submandibular gland protein C (Smgc)

MR219211 10 µg Ask for price

Lenti ORF clone of Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946)

MR201463L3 10 µg Ask for price

Lenti ORF clone of Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369)

MR203492L3 10 µg Ask for price

Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K

EF017179 96 Tests
EUR 826.8

Recombinant Rat Glandular kallikrein-7, submandibular/renal(Klk7)

AP70475 1mg
EUR 2826

Recombinant Rat Glandular kallikrein-7, submandibular/renal (Klk7)

CSB-EP012458RA 1553 mg Ask for price

Smgc (Myc-DDK-tagged ORF) - Rat submandibular gland protein C (Smgc), (10 ug)

RR212008 10 µg Ask for price

Recombinant Dog Double-headed protease inhibitor, submandibular gland

RPC27316-100ug 100ug
EUR 801.9

Recombinant Dog Double-headed protease inhibitor, submandibular gland

RPC27316-1mg 1mg
EUR 2885.2

Recombinant Dog Double-headed protease inhibitor, submandibular gland

RPC27316-20ug 20ug
EUR 448.1

Recombinant Dog Double-headed protease inhibitor, submandibular gland

CSB-EP360611DO 7287 mg Ask for price

Lenti ORF particles, Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946), 200ul, >10^7 T

MR201463L4V 200 µl Ask for price

Lenti ORF particles, Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369), 200ul, >10^7 T

MR203492L4V 200 µl Ask for price

Bpifa2f (untagged ORF) - Rat neonatal submandibular gland protein B (Smgb), (10 ug)

RN212284 10 µg Ask for price

Recombinant Rat Submandibular gland secretory Glx-rich protein CB (Grpcb)

CSB-YP357398RAa0 10597 mg Ask for price

Prol1 (untagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), (10 ug)

RN215454 10 µg Ask for price

Lenti ORF particles, Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946), 200ul, >10

MR201463L3V 200 µl Ask for price

Lenti ORF particles, Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369), 200ul, >10

MR203492L3V 200 µl Ask for price

Lenti ORF clone of Smgc (mGFP-tagged ORF) - Rat submandibular gland protein C (Smgc), (10 ug)

RR212008L4 10 µg Ask for price

Bpifa2f (Myc-DDK-tagged ORF) - Rat neonatal submandibular gland protein B (Smgb), (10 ug)

RR212284 10 µg Ask for price

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 ea Ask for price

Prol1 (Myc-DDK-tagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), (10 ug)

RR215454 10 µg Ask for price

Lenti ORF clone of Smgc (Myc-DDK-tagged ORF) - Rat submandibular gland protein C (Smgc), (10 ug)

RR212008L3 10 µg Ask for price

Lenti ORF particles, Smgc (GFP-tagged ORF) - Rat submandibular gland protein C (Smgc), 200ul, >10^7 TU/mL

RR212008L4V 200 µl Ask for price

Lenti ORF clone of Bpifa2f (mGFP-tagged ORF) - Rat neonatal submandibular gland protein B (Smgb), (10 ug)

RR212284L4 10 µg Ask for price

Lenti ORF clone of Prol1 (mGFP-tagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), (10 ug)

RR215454L4 10 µg Ask for price

Lenti ORF particles, Smgc (Myc-DDK-tagged ORF) - Rat submandibular gland protein C (Smgc), 200ul, >10^7 TU/mL

RR212008L3V 200 µl Ask for price

Lenti ORF clone of Bpifa2f (Myc-DDK-tagged ORF) - Rat neonatal submandibular gland protein B (Smgb), (10 ug)

RR212284L3 10 µg Ask for price

Lenti ORF clone of Prol1 (Myc-DDK-tagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), (10 ug)

RR215454L3 10 µg Ask for price

Lenti ORF particles, Bpifa2f (GFP-tagged ORF) - Rat neonatal submandibular gland protein B (Smgb), 200ul, >10^7 TU/mL

RR212284L4V 200 µl Ask for price

Lenti ORF particles, Prol1 (GFP-tagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), 200ul, >10^7 TU/mL

RR215454L4V 200 µl Ask for price

Lenti ORF particles, Bpifa2f (Myc-DDK-tagged ORF) - Rat neonatal submandibular gland protein B (Smgb), 200ul, >10^7 TU/mL

RR212284L3V 200 µl Ask for price

Lenti ORF particles, Prol1 (Myc-DDK-tagged ORF) - Rat mucin 10, submandibular gland salivary mucin (Muc10), 200ul, >10^7 TU/mL

RR215454L3V 200 µl Ask for price

Epithelial Dissociation System 2 (Epididymal epithelial, Gastric parietal and chief Submandibular salivary), Mouse and Rat

4-20252 ea Ask for price

Pig Salivary, Submaxillary cDNA

PD-317 30 reactions
EUR 243

Sheep Salivary, Submaxillary cDNA

SD-317 30 reactions
EUR 243

Bovine Salivary, Submaxillary cDNA

BD-317 30 reactions
EUR 243

Pig SUBMAXILLARY 50 EA*

59443-2 50 EA
EUR 493

Rabbit SALIVARY SUBMAX Y 50 EA*

41243-2 50 EA
EUR 382.07

MINI GEL-O SUBMARINE SYSTEM

JSB-30 7.5CM X 10CM FORMAT
EUR 549.58

LARGE GEL-O SUBMARINE SYSTEM

JSB-120 -
EUR 920.57

ULTRAWIDE MINI SUBMARINE

JSB-96 SYSTEM 23.5CM X 15CM FORMAT
EUR 872.06

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

Rabbit SUBMAXILLARY MATURE 50 EA

41343-2 50 EA
EUR 382.07

Oak cDNA

PLD-1005 30 reactions
EUR 498

Fig cDNA

PLD-1042 30 reactions
EUR 498

Oat cDNA

PLD-1096 30 reactions
EUR 498

Rye cDNA

PLD-1097 30 reactions
EUR 498

Corn cDNA

PLD-1002 30 reactions
EUR 498

Rice cDNA

PLD-1004 30 reactions
EUR 498

Pear cDNA

PLD-1033 30 reactions
EUR 498

Pine cDNA

PLD-1063 30 reactions
EUR 498

DOUBLEWIDE MINI GELO SUBMARINE

JSB-302 SYSTEM 15CM X 10CM FORMAT
EUR 725.15

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

Apple cDNA

PLD-1001 30 reactions
EUR 498

Beans cDNA

PLD-1051 30 reactions
EUR 498

Grape cDNA

PLD-1052 30 reactions
EUR 498

Lemon cDNA

PLD-1062 30 reactions
EUR 498

Wheat cDNA

PLD-1084 30 reactions
EUR 498

Onion cDNA

PLD-1092 30 reactions
EUR 498

Maple cDNA

PLD-1094 30 reactions
EUR 498

Orange cDNA

PLD-1003 30 reactions
EUR 498

Cotton cDNA

PLD-1022 30 reactions
EUR 498

Banana cDNA

PLD-1031 30 reactions
EUR 498

Barley cDNA

PLD-1041 30 reactions
EUR 498

Pepper cDNA

PLD-1043 30 reactions
EUR 498

Potato cDNA

PLD-1073 30 reactions
EUR 498

Tomato cDNA

PLD-1074 30 reactions
EUR 498

Carrot cDNA

PLD-1081 30 reactions
EUR 498

Radish cDNA

PLD-1083 30 reactions
EUR 498

Cherry cDNA

PLD-1091 30 reactions
EUR 498

Squash cDNA

PLD-1111 30 reactions
EUR 498

Bovine SALIVARY SUBMAXILLARY 50 EA*

57143-2 50 EA
EUR 555.64

OORA00346-10U - SUBMAXILLARY

OORA00346-10U 1Each
EUR 120

OORA00346-1EA - SUBMAXILLARY

OORA00346-1EA 1Each
EUR 119

OORA00488-10U - SUBMAXILLARY

OORA00488-10U 1Each
EUR 55

OORA00488-1EA - SUBMAXILLARY

OORA00488-1EA 1Each
EUR 59

OORA00547-1U - Rat SUBMAXILLARY

OORA00547-1U 1Each
EUR 60

Apricot cDNA

PLD-1011 30 reactions
EUR 498

Soybean cDNA

PLD-1044 30 reactions
EUR 498

Spinach cDNA

PLD-1054 30 reactions
EUR 498

Cabbage cDNA

PLD-1071 30 reactions
EUR 498

Lettuce cDNA

PLD-1072 30 reactions
EUR 498

Tobacco cDNA

PLD-1101 30 reactions
EUR 498

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

Cucumber cDNA

PLD-1032 30 reactions
EUR 498

Broccoli cDNA

PLD-1061 30 reactions
EUR 498

Rapeseed cDNA

PLD-1093 30 reactions
EUR 498

Flaxseed cDNA

PLD-1095 30 reactions
EUR 498

OORA00547-1EA - Rat SUBMAXILLARY

OORA00547-1EA 1Each
EUR 59

Pig Salivary, Submaxillary Total RNA

PR-317 0.1mg
EUR 160

Persimmon cDNA

PLD-1053 30 reactions
EUR 498

Sunflower cDNA

PLD-1064 30 reactions
EUR 498

Nectarine cDNA

PLD-1082 30 reactions
EUR 498

Asparagus cDNA

PLD-1131 30 reactions
EUR 498

Arabidopsis cDNA

PLD-1021 30 reactions
EUR 498

OORA00518-1U - Rabbit SUBMAXILLARY

OORA00518-1U 1Each
EUR 255

Sheep Salivary, Submaxillary Total RNA

SR-317 0.1mg
EUR 160

OORA00518-1EA - Rabbit SUBMAXILLARY

OORA00518-1EA 1Each
EUR 239

Bovine Salivary, Submaxillary Total RNA

BR-317 0.1mg
EUR 160

OORA00435-10U - GUINEA PIG SUBMAXILLARY

OORA00435-10U 1Each
EUR 80

OORA00435-1EA - GUINEA PIG SUBMAXILLARY

OORA00435-1EA 1Each
EUR 79

OORA00465-10U - Hamster SUBMAXILLARY

OORA00465-10U 1Each
EUR 75

OORA00465-1EA - Hamster SUBMAXILLARY

OORA00465-1EA 1Each
EUR 69

Pig Salivary, Submaxillary Total Protein

PT-317 1mg
EUR 153

Rat Eye cDNA

RD-106 30 reactions
EUR 243

Dog Eye cDNA

DD-106 30 Reactions
EUR 280

Cat Eye cDNA

FD-106 30 Reactions
EUR 280

Novo? cDNA Kit

M1165-100 each
EUR 424.8

Novo? cDNA Kit

M1165-25 each
EUR 320.4

Pig Skin cDNA

PD-101 30 reactions
EUR 243

Pig Lung cDNA

PD-601 30 reactions
EUR 243

Rat Skin cDNA

RD-101 30 reactions
EUR 243

Rat Pons cDNA

RD-207 30 reactions
EUR 243

Rat Lung cDNA

RD-601 30 reactions
EUR 243

Dog Skin cDNA

DD-101 30 Reactions
EUR 280

Dog Lung cDNA

DD-601 30 Reactions
EUR 280

Cat Lung cDNA

FD-601 30 Reactions
EUR 280

Pig Salivary, Submaxillary Frozen Sections

PF-317 10 slides
EUR 261
Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Major histocompatibility complicated (MHC) represents an essential genetic marker for manipulation to enhance the well being and productiveness of cattle. It is intently related to quite a few illness susceptibilities and immune responses. Bovine MHC, additionally known as bovine leukocyte antigen (BoLA), is taken into account as an acceptable marker for genetic variety research. In cattle, most of the polymorphisms are positioned in exon 2 of BoLA-DRB3, which encodes the peptide-binding cleft. In this research, the polymorphism of the BoLA-DRB3.2 gene in Holstein’s calves was studied utilizing excessive decision melting curve analysis (HRM).

Observed HRM outcomes have been in comparison with PCR-RFLP and direct sequencing strategies. Eight completely different HRM and seven completely different RFLP profiles have been recognized among the many inhabitants studied. By evaluating to sequencing knowledge, HRM may fully discriminate all genotypes (eight profiles), whereas the RFLP failed to differentiate between the genotypes *1101/*1001 and *1104/*1501. According to the outcomes, the HRM analysis methodology gave extra correct outcomes than RFLP by differentiating between the BoLA-DRB3.2 genotypes.

Due to the Co-dominant nature of the MHC alleles, HRM method might be used for investigating the polymorphisms of genotypes and their associations with immune responses. In transfection experiments with mammalian cells aiming to overexpress a selected protein, it’s usually essential to appropriately quantify the extent of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts have been transfected with a vector containing the whole Pex11β cDNA (plasmid DNA).

The Pex11β mRNA stage, as calculated utilizing the RT-qPCR product, was unrealistically larger (>1000-fold) in transfected in comparison with non-transfected cells, and we assumed that there have been giant quantities of contaminating plasmid DNA within the RNA pattern. Thus, we looked for a easy method to distinguish between plasmid-derived mRNA, endogenous genome-derived mRNA and plasmid DNA, with minimal adjustments to straightforward RT-PCR strategies.

Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis.

Epidermal development issue receptor (EGFR) mutation testing in plasma cell-free DNA (cfDNA) from superior lung most cancers sufferers is an rising scientific software. This meta-analysis was designed to find out the diagnostic accuracy of two widespread PCR programs, droplet digital PCR (ddPCR) and amplification refractory mutation system PCR (ARMS-PCR), for detecting EGFR mutation in cfDNA. A scientific search was carried out primarily based on PubMed, Web of science, Embase and the Cochrane library. Data from eligible research have been extracted and pooled to calculate the sensitivity, specificity, diagnostic odds ratio (DOR), space below the abstract receiver-operating attribute curve (AUROC), utilizing tissue biopsy outcomes as the usual methodology.

Subgroup analyses have been carried out relating to EGFR mutation kind, tumor stage, and EGFR-TKI remedy. Twenty-five research involving 4,881 circumstances have been included. The plasma testing sensitivity, specificity, DOR, and AUROC, in contrast with the matched tumor tissues, have been 72.1%, 95.6%, 38.5, 0.89 for ddPCR, and 65.3%, 98.2%, 52.8, 0.71 for ARMS-PCR, respectively, by oblique comparability, vital variations have been present in sensitivity (P = 0.003) and specificity (P = 0.007).

Furthermore, vital distinction was present in sensitivity between tumor stage subgroups (IIIB-IV subgroup vs. IA-IV subgroup) in ARMS-PCR (73.7 vs. 64.2%, P = 0.008), however not in ddPCR (72.5 vs. 71.2%, P = 0.756). This research demonstrates that ddPCR and ARMS-PCR have a excessive specificity with a sensible sensitivity for detecting EGFR mutation in cfDNA, which helps their utility as a complement or a conditional-alternative to tissue biopsy in scientific follow for genotyping. It appears that ddPCR has the next sensitivity than ARMS-PCR, particularly in early phases.

Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity analysis and complementing forensic surveillance.

Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is important for the prosecution of illegally-traded wildlife merchandise, conservation-based biodiversity analysis, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue depends on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which stays pricey for functions of screening giant numbers of unknown samples throughout routine surveillance.

Here, we tailored a fast, low-cost strategy to distinguish 10 home and 24 wildlife species which might be widespread within the East African unlawful wildlife merchandise commerce primarily based on their distinctive high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR merchandise. Using the strategy, we recognized (i) giraffe amongst covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences amongst savannah elephant reference samples. This strategy is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.

Universal DNA Marker (100 bp ~ 10,000 bp)

W2583-500 - Ask for price

QPCR Kit DNA Universal f

MOL6606 EACH
EUR 1118.9

QPCR Kit DNA Universal m

MOL6610 EACH
EUR 1118.9

QPCR Kit DNA Universal f Mmix

MOL6608 EACH
EUR 1406.13

QPCR Kit DNA Universal m Mmix

MOL6612 EACH
EUR 1406.13

Genorise® Universal DNA Mini Kit 50

GR101076 50
EUR 223

Genorise® Universal DNA Mini Kit 100

GR101076-100 100
EUR 357

Genorise® Universal DNA Mini Kit 200

GR101077 200
EUR 567

Genorise® Universal DNA Mini Kit 500

GR101077-500 500
EUR 1272

Methylamp Universal Methylated DNA Kit 

P-1011
  • EUR 684.66
  • EUR 292.60
  • EUR 442.20
  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-02 96 rxn
EUR 2563.2

VAHTS Universal DNA library Prep Kit for MGI V4

NDM610-01 24 rxns
EUR 235.2

VAHTS Universal DNA library Prep Kit for MGI V4

NDM610-02 96 rxns
EUR 880

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-01 24 rxn
EUR 529.2

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-02 96 rxn
EUR 1686

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-02 96 rxn
EUR 2563.2

VAHTS Universal DNA Library Prep Kit for Illumina V4

ND610-01 24 rxns
EUR 235.2

VAHTS Universal DNA Library Prep Kit for Illumina V4

ND610-02 96 rxns
EUR 880

AFTSpin Universal Plant Fast DNA Extraction Kit

RK30111 50RXN
EUR 69

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

ND702-01 24 rxns
EUR 235.2

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

ND702-02 96 rxns
EUR 880

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-01 24 rxn
EUR 907.2

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-02 96 rxn
EUR 2740.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-01 24 rxn
EUR 988.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-02 96 rxn
EUR 2889.6

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-01 24 rxn
EUR 558

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-02 96 rxn
EUR 1858.8

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

NDM627-01 24 rxns
EUR 250

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

NDM627-02 96 rxns
EUR 920

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-01 24 rxn
EUR 590.4

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-02 96 rxn
EUR 1926

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-03 24 rxn
EUR 514.8

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-04 96 rxn
EUR 1624.8

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

ND627-01 24 rxns
EUR 250

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

ND627-02 96 rxns
EUR 920

Universal platform

INC1112 EACH
EUR 1434

Universal Platform

SS-300-U each
EUR 250

Universal Attachment

MIX1320 EACH
EUR 286.8

Universal V1-Puck

M-CP-111-021 1 PUCK
EUR 350
Description: Universal V1-Puck

Universal Oven UN30

OVE8314 EACH
EUR 1672.8

Universal Oven UN55

OVE8316 EACH
EUR 1952.4

Universal Oven UN75

OVE8318 EACH
EUR 2028

Universal Oven UF30

OVE8332 EACH
EUR 1911.6

Universal Oven UF55

OVE8334 EACH
EUR 2306.4

Universal Oven UF75

OVE8336 EACH
EUR 2433.6

UNIVERSAL BEER AGAR

U21-100-10kg 10 kg
EUR 1828.8

UNIVERSAL BEER AGAR

U21-100-2kg 2kg
EUR 444

UNIVERSAL BEER AGAR

U21-100-500g 500 g
EUR 164.4

Grant Universal Tray

BAT4622 EACH
EUR 314.4

Universal AC adapter

PIP3214 EACH
EUR 78

Universal Oven UN110

OVE8320 EACH
EUR 2598

Universal Oven UN160

OVE8322 EACH
EUR 3133.2

Universal Oven UN260

OVE8324 EACH
EUR 4148.4

Universal Oven UN450

OVE8326 EACH
EUR 5490

Universal Oven UN750

OVE8328 EACH
EUR 7356

Universal Oven UF110

OVE8338 EACH
EUR 2876.4

Universal Oven UF160

OVE8340 EACH
EUR 3740.4

Universal Oven UF260

OVE8342 EACH
EUR 4768.8

Universal Oven UF450

OVE8344 EACH
EUR 6244.8

Universal Oven UF750

OVE8346 EACH
EUR 8275.2

Universal WB Toolkit

KTD106-EN-20T 20 T
EUR 59
Description: Abbkine has developed a Universal WB Toolkit. After careful design and research, one-stop operation can meet the needs of routine WB experiments and obtain excellent experimental results.

Universal Spring Tray

BAT7177 EACH
EUR 298.8

Universal Oven UF1060

OVE8348 EACH
EUR 15282

NBS Universal Platform

SHA4050 EACH
EUR 1459.2

NBS Universal Platform

SHA4070 EACH
EUR 1059.6

NBS Universal Platform

SHA4232 EACH
EUR 580.8

NBS Universal Platform

SHA4256 EACH
EUR 711.6

NBS Universal Platform

SHA4272 EACH
EUR 1129.2

NBS Universal Platform

SHA4290 EACH
EUR 1519.2

Universal Block Buffer

TBS5013 100 ml
EUR 60

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 331.44

Mains adaptor (universal)

ARG1248 EACH
EUR 135.78

Universal Oven UN30plus

OVE8350 EACH
EUR 2390.4

Universal Oven UN55plus

OVE8352 EACH
EUR 2608.8

Universal Oven UN75plus

OVE8354 EACH
EUR 2902.8

Universal Oven UF30plus

OVE8366 EACH
EUR 2523.6

Universal Oven UF55plus

OVE8368 EACH
EUR 2752.8

Universal Oven UF75plus

OVE8370 EACH
EUR 3300

Loopster Universal Disc

MIX1080 EACH
EUR 180

Universal Oven UN110plus

OVE8356 EACH
EUR 3327.6

Universal Oven UN260plus

OVE8358 EACH
EUR 4956

Universal Oven UN450plus

OVE8360 EACH
EUR 6390

Universal Oven UN750plus

OVE8362 EACH
EUR 8228.4

Universal Oven UF110plus

OVE8372 EACH
EUR 3807.6

Universal Oven UF160plus

OVE8374 EACH
EUR 4743.6

Universal Oven UF260plus

OVE8376 EACH
EUR 5660.4

Universal Oven UF450plus

OVE8378 EACH
EUR 7177.2

Universal Oven UF750plus

OVE8380 EACH
EUR 9790.8

IKA Universal Attachment

SHA2016 EACH
EUR 997.2

Universal attachment IKA

SHA2210 EACH
EUR 783.6

Universal Stratus Adapter

MIC6036 EACH
EUR 592.8

Universal Oven UF1060plus

OVE8382 EACH
EUR 16224

Universal Spring Platform

MIX7184 EACH
EUR 885.6

Universal Spring Platform

H1000-P-SP 1 PC
EUR 640.9

Universal Spring Platform

H1010-P-SP 1 each
EUR 677.5

Universal Ohaus Plug GB

46001779 EACH
EUR 16.8

18mmMag Universal ScrewCap

CHR3460 PK1000
EUR 1339.2

Universal tray for LSB12

BAT1062 EACH
EUR 300

Universal tray for LSB18

BAT1064 EACH
EUR 314.4

Azo Universal spray 500ml

CLE1996 EACH
EUR 10.29

2 Universal Clip Plates

MIX5190 EACH
EUR 110.4

Universal (Aluminum) Platform

MIX1322 EACH
EUR 139.2

Universal Multichannel Stand

F161417 EACH
EUR 33.6

Universal Indicator Solution

UN10025 2.5L
EUR 205.2

Universal Indicator Solution

UN1005 50ML
EUR 21.31

Universal Indicator Solution

UN101 100ML
EUR 22.8

Universal Indicator Solution

UN1025 25L
EUR 812.08

Universal Indicator Solution

UN105 500ML
EUR 111.6

Universal Indicator Solution

UNB1010 10L
EUR 782.05

Universal Restriction Buffer

EN-300 5 x 1ml
EUR 22.7

PCR plate-foil universal

A28216 1x100 pcs
EUR 112.8

PCR plate-foil universal

A28216L 5x100 pcs
EUR 372

AED Universal Wall Bracket

SAF1128 EACH
EUR 107.06

Ilford Universal Stop Bath

21956-500 500ml
EUR 73

Universal Detachment Solution

AD002 100 ml
EUR 177.6

UNIVERSAL PREENRICHMENT BROTH

U21-101-10kg 10 kg
EUR 898.8

UNIVERSAL PREENRICHMENT BROTH

U21-101-2Kg 2 Kg
EUR 242.4

UNIVERSAL PREENRICHMENT BROTH

U21-101-500g 500 g
EUR 109.2

Wire Rack 2x 6 Universal

STA20020 EACH
EUR 36

5min Universal Ligation Mix

C311-01 50 rxn
EUR 256.8

5min Universal Ligation Mix

C311-02 100 rxn
EUR 339.6

18mm Mag Universal ScrewCap

CHR3466 PK1000
EUR 1266

18mm Mag Universal ScrewCap

CHR3468 PK1000
EUR 954

Pack of 10 universal seals

WAT4870 PK10
EUR 214.12

Universal Nuclease ELISA Kit

RK04660 96T
EUR 280

Universal Neutralization Buffer

NB001 100 ml
EUR 162

Universal V1-Puck; Qnty 7

M-CP-111-021-7 7 PUCKS
EUR 2420
Description: Universal V1-Puck; Qnty 7

10 × Universal Primer Mix (UPM)

RA102-01 100 rxns (5 μl/rxn)
EUR 58

Glass universal 30ml sep cap

127030CS PK500
EUR 246

Universal Indicator pH 1-14

RRSP4455-D 500ml
EUR 8.63

Universal Indicator pH 1-14

RRSP4455-E 1L
EUR 15.86

Universal Indicator pH 1-14

RRSP4455-F 2.5L
EUR 30.22

Universal Platform Innova S44i

SHA3072 EACH
EUR 1449.6

Nunc Universal Lid PS Sterile

250002 PK60
EUR 294

Universal Platform 28 X 33cm

SHA6028 EACH
EUR 223.2

Universal Platform 33 X 33cm

SHA6032 EACH
EUR 319.2

The mouse transplantation mannequin stays essentially the most related methodology to evaluate the practical capacities of mammary cells and is especially applicable for investigations relating to mammary stem cells, regardless of the species studied. Following xenotransplantation in mice mammary fats pad, the event of the xenograft is usually evaluated by immunohistology. Here, we current a easy and fast methodology to manage the species specificity of a xenograft primarily based on genomic DNA PCR amplification. DNA is extracted from the mounted samples meant for histology, thus permitting the reuse of treasured samples. Standard and digital droplet PCR (requiring low DNA portions) strategies have been used to make the current methodology appropriate for the analysis of xenotransplanted samples.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

Overwhelming proof means that in addition to the genetic adjustments of DNA mutations, epigenetic adjustments of DNA methylation and histone modifications play vital position in regulation of gene expression. DNA methylation is essentially the most frequent epigenetic alteration noticed in mammalian genomes, and usually it’s negatively correlated with gene expression. Various strategies can be found for the detection of DNA methylation adjustments. Although the current high-throughput strategies for DNA methylation evaluation have varied benefits, they require excessive ranges of technical experience, pricey gear, and reagents.

Because of these causes, many of the worldwide DNA methylation evaluation strategies are primarily carried out at core facility, and laboratories with restricted assets and experience are usually not ready to make use of these strategies. Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) is a restriction enzyme digestion and PCR-based technique for the evaluation of DNA methylation adjustments.

This technique is cost-effective, requires easy and fundamental instrumentation, and due to this fact can simply be carried out in any laboratory with fundamental setup having an everyday DNA thermal cycler and DNA gel electrophoresis system. Additional benefits of this technique over different strategies for DNA methylation evaluation are that it requires very much less quantity of DNA and might display DNA methylation adjustments globally at genome-wide stage with excessive sensitivity. This technique has been efficiently used to detect adjustments in DNA methylation both occurring naturally or induced by varied toxicants and environmental components. A element experimental protocol for MS-RAPD-PCR is described in this chapter.

Laphet is a standard fermented meals in Myanmar, constructed from tea leaves (Camellia sinensis) by fermentation with restricted air passage. We carried out microbial range analyses on 14 Laphet merchandise collected from totally different places in Myanmar. Amplicon-based sequencing outcomes revealed Lactobacillus and Acetobacter had been plentiful micro organism and Candida, Pichia, Cyberlindnera, and Debaryomyces had been plentiful yeast. Using selective media, eight species of lactic acid micro organism and 9 species of yeast had been remoted; Lactobacillus plantarum and L. collinoides had been dominant micro organism and Pichia manshurica, Candida boidinii, and Cyberlindnera jadinii had been main yeasts. PCR-DGGE evaluation confirmed that almost all of the dominant bacterial and yeast species discovered in tradition dependent evaluation had been current in Laphet samples.

High density DNA methylation array is a dependable various for PCR-based evaluation of the MGMT promoter methylation standing in glioblastoma.

MGMT promoter methylation standing is a crucial biomarker predicting survival and response to chemotherapy in sufferers affected by glioblastoma. Since new diagnostic strategies akin to methylome-based classification of mind tumors are increasingly continuously carried out, we geared toward evaluating the suitability of calculating the MGMT promoter methylation standing in a quantitative method from the methylome profiling as in comparison with the traditional gold normal evaluation by PCR.Our cohort consisted of 39 instances identified as “glioblastoma, IDH-wildtype” of which the MGMT promoter methylation standing was analyzed with each methylation-specific PCR and excessive density DNA methylation array utilizing the STP-27 algorithm.

Contradictory outcomes had been validated by pyrosequencing.The inter-method reliability reached 77% (kappa-coefficient: 0.58) when additionally instances with an inconclusive end result in one or the opposite technique had been taken into consideration. When solely instances with conclusive outcomes in each strategies had been thought of, a really excessive inter-method reliability of 91% (kappa-coefficient: 0.86) could possibly be achieved. For “methylated” instances, no contradictory outcomes had been obtained.

For the remaining two instances with discrepant outcomes subsequent pyrosequencing analyses spoke in favor of every beforehand utilized technique as soon as.In addition to its advantages for molecular subgrouping and duplicate quantity evaluation of mind tumors, DNA-methylation based mostly classification is a extremely dependable instrument for the evaluation of MGMT promoter methylation standing in glioblastoma sufferers.

Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive-Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).

PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis.

Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been a staple of analysis, offering valuable assets for molecular and genomic research. However, the most important problem is the extraction of high-quality DNA from FFPE tissues, on condition that the integrity of DNA is critically affected by formalin fixation. Formaldehyde induces crosslinks in DNA that renders single or double-stranded DNA breaks. Such breaks trigger in depth fragmentation that instantly influences the standard of DNA purified and the quantity of templates out there for PCR amplification. Thus, protocol for DNA purification from FFPE tissues should successfully extract extremely fragmented DNA and reverse cross-linking brought about by formalin fixation.

DNA extraction strategies out there in the literature had been chosen and modified at totally different phases to optimize a protocol that extracts DNA of adequate high quality and fragment dimension to be detectable by PCR. Archived FFPE tissues belonged to sufferers with triple damaging breast most cancers (TNBC) and benign breast illness had been used for the protocol optimization. The finest optimized protocol was then used to amplify Exon four area of Proviral integration web site for Moloney murine leukemia virus1 (Pim1) kinase gene to research any possible somatic mutations each in TNBCs and benign breast illnesses.

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 385

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 385

Total RNA - Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 130

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 286

Rat normal kidney tissue array

TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 530

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 130

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 286

Total Protein - Mouse Normal Tissue: Kidney

P1334142 1 mg
EUR 188

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array

KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 170

Kidney cancer tissue array with normal tissues

KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues

KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney

R1534142-50 50 ug
EUR 191

Kidney tumor tissue array with normal tissue as control

KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney

P1234142 1 mg
EUR 188

Kidney cancer tissue array with normal tissue as control

KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney

R1534142-Cy 50 ug
EUR 191

Adjacent normal kidney tissue and cancer tissue array

BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney

P3234142 0.1 mg
EUR 270

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney

R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue

KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue

Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Monkey (Cyno.) Normal Kidney Whole tissue lysate

MCL-1190 1 mg
EUR 628.8

Human Adult Kidney (Normal) Whole tissue lysate

HAL-1305 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate

MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 343

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues

Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Paraffin Tissue Section - Human Adult Normal: Kidney

T2234142 5 slides
EUR 185

Multiple Kidney cancer tissue array with normal tissue control from autopsy

KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 416

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts

9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney

R1234142-P 50 ug
EUR 370

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)

RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack

TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor

D1234142 100 ug
EUR 286

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 463

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes

9-80172 1 x 100 ml Ask for price

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue

KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 385

Kidney cancer with matched cancer adjacent normal tissue test array

KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array

KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 385

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80262 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 385

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 663

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 578

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes

9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells

9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts

9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts

9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma

KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack

TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack

TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells

9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells

9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 578

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)

TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array

KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80170 1 x 100 ml Ask for price

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 SLIDES/PACK)

NCT176 5 slides
EUR 300

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 583

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells

9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells

9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 375

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 385

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-1 1 slide
EUR 692

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-5 5 slides
EUR 2273

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 385

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

Kidney Tumor Tissue Array - Duplicated 70 cases covering all the common types of kidney cancer and 5 cases of normal and

Z7020052 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Kidney Tumor Tissue Array - Duplicated 36 cases covering all the common types of kidney cancer and 12 cases of normal an

Z7020054 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA - Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 385

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

cDNA - Human Diabetic Diseased Tissue: Kidney

C1236142Dia 40 reactions
EUR 729

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

cDNA - Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 578

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 215

cDNA - Monkey (Rhesus) Normal Tissue: Lung

C1534152 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Skin

C1534218 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 385

Of the 12 totally different protocols developed, highest quality DNA in phrases of fragment dimension and purity was obtained when Tween20 lysis buffer was used for each deparaffinization and in a single day digestion together with excessive salt precipitation. Optimized protocol was then validated by extracting DNAs from 10 TNBCs and 5 benign breast illness specimens with constant purity and fragment dimension. PCR amplification and subsequent Sanger’s sequencing revealed the presence of mutations in the Exon four area of Pim1 kinase. Deparaffinization and in a single day digestion in Tween20 lysis buffer together with excessive salt precipitation yielded the highest quality PCR amplifiable DNA for mutational evaluation.